Rabbit anti-Human PRSS8 Polyclonal Antibody | anti-PRSS8 antibody

PRSS8, ID (PRSS8, Prostasin, Serine protease 8, Prostasin light chain, Prostasin heavy chain) (AP)

Cat. Num. AAA6337974

• Gene Names: PRSS8; CAP1; PROSTASIN
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PRSS8; Polyclonal Antibody; ID (PRSS8; Prostasin; Serine protease 8; Prostasin light chain; Prostasin heavy chain) (AP); Prostasin heavy chain; anti-PRSS8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRSS8.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PRSS8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 190-219 in the central region of human PRSS8, UniProt Accession #Q16651.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB453 cell line lysates (35ug/lane) usingMBS6011679. This demonstrates that MBS6011679 detected the PRSS8 protein (arrow).)

Related Product Information for anti-PRSS8 antibody

This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

Product Categories/Family for anti-PRSS8 antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

1. Chen, Y.W., et al. J. Biol. Chem. 285(41):31755-31762(2010). 2. Chen, M., et al. Mol. Cell. Biochem. 337 (1-2), 259-266 (2010). 3. Fu, Y.Y., et al. Hum. Reprod. 25(3):623-632(2010). 4. Ko, T., et al. J. Biomed. Biotechnol. 2010, 793843 (2010). 5. Chang, J.H., et al. Zhongguo Yi Xue Ke Xue Yuan Xue Bao 31(6):712-719(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4506153
• NCBI GeneID: 5652
• NCBI Accession #: NP_002764.1
• NCBI GenBank Nucleotide #: NM_002773.4
• UniProt Accession #: Q16651; Q9UCA3; B4DWP2
• Molecular Weight: 30,519 Da
• NCBI Official Full Name: prostasin preproprotein
• NCBI Official Synonym Full Names: protease, serine 8
• NCBI Official Symbol: PRSS8
• NCBI Official Synonym Symbols: CAP1; PROSTASIN
• NCBI Protein Information: prostasin
• UniProt Protein Name: Prostasin
• Protein Family: Prostasin
• UniProt Gene Name: PRSS8
• UniProt Synonym Gene Names: CAP1
• UniProt Entry Name: PRSS8_HUMAN

NCBI Description

This gene encodes a member of the peptidase S1 or chymotrypsin family of serine proteases. The encoded preproprotein is proteolytically processed to generate light and heavy chains that associate via a disulfide bond to form the heterodimeric enzyme. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. This protease exhibits trypsin-like substrate specificity, cleaving protein substrates at the carboxyl terminus of lysine or arginine residues. The encoded protease partially mediates proteolytic activation of the epithelial sodium channel, a regulator of sodium balance, and may also play a role in epithelial barrier formation. [provided by RefSeq, Feb 2016]

Uniprot Description

PRSS8: Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G). Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.-; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: extracellular space; integral to membrane; extracellular region; plasma membrane

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis