Typical Testing Data/Standard Curve (for reference only)
Mouse KIM1 ELISA Kit | HAVCR1 elisa kit
Mouse KIM1 PicoKine ELISA Kit
Gene Names
Havcr1; Tim1; KIM-1; TIM-1; Timd1; AI503787
Reactivity
Mouse
Synonyms
KIM1; Mouse KIM1 PicoKine ELISA Kit; Hepatitis A virus cellular receptor 1; hepatitis A virus cellular receptor 1; HAVCR; HAVcr-1; Havcr1; HAVR1_HUMAN; Kidney injury molecule 1; KIM-1; T cell immunoglobin domain and mucin domain protein 1; T-cell immunoglobulin and mucin domain-containing protein 1; T-cell membrane protein 1; TIM; TIM-1; TIM1; TIMD-1; TIMD1; HAVCR1 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Havcr1
Sequence Length
282
Samples
Cell culture supernatants, serum, plasma (heparin, edta) and urine
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<2 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HAVCR1 elisa kit
Principle of the Assay: Picokine™ Mouse Havcr1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Havcr1 with a 96-well strip plate that is pre-coated with antibody specific for Havcr1. The detection antibody is a biotinylated antibody specific for Havcr1. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Havcr1 with immunogen: Expression system for standard: NS0; Immunogen sequence: Y22-T212. The kit is analytically validated with ready-to-use reagents. To measure Mouse Havcr1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Havcr1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Havcr1 in the sample.
Background/Introduction: KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.
Background/Introduction: KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,968 Da
NCBI Official Full Name
11 days pregnant adult female ovary and uterus cDNA, RIKEN full-length enriched library, clone:5031400I12 product:hepatitis A virus cellular receptor 1, full insert sequence
NCBI Official Synonym Full Names
hepatitis A virus cellular receptor 1
NCBI Official Symbol
Havcr1
NCBI Official Synonym Symbols
Tim1; KIM-1; TIM-1; Timd1; AI503787
NCBI Protein Information
hepatitis A virus cellular receptor 1 homolog; TIMD-1; HAVcr-1; kidney injury molecule 1; t cell membrane protein 1; T-cell immunoglobulin mucin receptor 1; T-cell immunoglobulin and mucin domain containing 1; t cell immunoglobulin and mucin domain-containing protein 1
UniProt Protein Name
Hepatitis A virus cellular receptor 1 homolog
Protein Family
UniProt Gene Name
Havcr1
UniProt Entry Name
Q3V033_MOUSE
Uniprot Description
TIM-1: May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. Belongs to the immunoglobulin superfamily. TIM family.
Protein type: Membrane protein, integral
Cellular Component: membrane; integral to membrane
Molecular Function: protein binding
Biological Process: positive regulation of mast cell activation
Research Articles on HAVCR1
Similar Products
Product Notes
The Mouse HAVCR1 havcr1 (Catalog #AAA175828) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175828 ELISA Kit recognizes Mouse HAVCR1. It is sometimes possible for the material contained within the vial of "KIM1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
