Typical Testing Data/Standard Curve (for reference only)
Rabbit Glutamate Receptor, Ionotropic, Delta 2 Interacting Protein ELISA Kit | GRId2IP elisa kit
Rabbit Glutamate Receptor, Ionotropic, Delta 2 Interacting Protein ELISA Kit
Reactivity
Rabbit
Synonyms
Glutamate Receptor; Ionotropic; Delta 2 Interacting Protein; Rabbit Glutamate Receptor; Delta 2 Interacting Protein ELISA Kit; GRId2IP elisa kit
Reactivity
Rabbit
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GRId2IP elisa kit
Intended Uses: This GRId2IP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit GRId2IP. This ELISA kit for research use only!
Principle of the Assay: GRId2IP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRId2IP antibody and an GRId2IP-HRP conjugate. The assay sample and buffer are incubated together with GRId2IP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRId2IP concentration since GRId2IP from samples and GRId2IP-HRP conjugate compete for the anti-GRId2IP antibody binding site. Since the number of sites is limited, as more sites are occupied by GRId2IP from the sample, fewer sites are left to bind GRId2IP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRId2IP concentration in each sample is interpolated from this standard curve.
Principle of the Assay: GRId2IP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRId2IP antibody and an GRId2IP-HRP conjugate. The assay sample and buffer are incubated together with GRId2IP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRId2IP concentration since GRId2IP from samples and GRId2IP-HRP conjugate compete for the anti-GRId2IP antibody binding site. Since the number of sites is limited, as more sites are occupied by GRId2IP from the sample, fewer sites are left to bind GRId2IP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRId2IP concentration in each sample is interpolated from this standard curve.
Product Categories/Family for GRId2IP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
113,535 Da
NCBI Official Full Name
Grid2ip protein
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, delta 2 (Grid2) interacting protein 1
NCBI Official Symbol
Grid2ip
NCBI Protein Information
delphilin; L-delphilin; GluR-delta2 philic-protein; glutamate receptor, ionotropic, delta 2-interacting protein 1
UniProt Protein Name
Delphilin
Protein Family
UniProt Gene Name
Grid2ip
UniProt Entry Name
GRD2I_MOUSE
Uniprot Description
GRID2IP: Postsynaptic scaffolding protein at the parallel fiber- Purkinje cell synapse, where it may serve to link GRID2 with actin cytoskeleton and various signaling molecules.
Protein type: Adaptor/scaffold
Cellular Component: postsynaptic membrane; cell projection; membrane; dendritic spine; plasma membrane; synapse; cell junction
Molecular Function: protein binding
Biological Process: metabotropic glutamate receptor signaling pathway; actin cytoskeleton organization and biogenesis
Research Articles on GRId2IP
Similar Products
Product Notes
The Rabbit GRId2IP grid2ip (Catalog #AAA744448) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA744448 ELISA Kit recognizes Rabbit GRId2IP. It is sometimes possible for the material contained within the vial of "Glutamate Receptor, Ionotropic, Delta 2 Interacting Protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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