Sheep Aryl Hydrocarbon Receptor Interacting Protein ELISA Kit | AIP elisa kit

Sheep Aryl Hydrocarbon Receptor Interacting Protein ELISA Kit

Cat. Num. AAA751857

• Reactivity: Sheep
• Synonyms: Aryl Hydrocarbon Receptor Interacting Protein; Sheep Aryl Hydrocarbon Receptor Interacting Protein ELISA Kit; AIP elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AIP elisa kit

Principle of the assay: AIP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AIP antibody and an AIP-HRP conjugate. The assay sample and buffer are incubated together with AIP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AIP concentration since AIP from samples and AIP-HRP conjugate compete for the anti-AIP antibody binding site. Since the number of sites is limited, as more sites are occupied by AIP from the sample, fewer sites are left to bind AIP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AIP concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 224177838
• Molecular Weight: 37,690 Da
• NCBI Official Full Name: aryl hydrocarbon receptor interacting protein
• UniProt Protein Name: Aryl hydrocarbon receptor interacting protein
• Protein Family: AH receptor-interacting protein
• UniProt Gene Name: AIP
• UniProt Entry Name: C0JXB9_HUMAN

NCBI Description

The protein encoded by this gene is a receptor for aryl hydrocarbons and a ligand-activated transcription factor. The encoded protein is found in the cytoplasm as part of a multiprotein complex, but upon binding of ligand is transported to the nucleus. This protein can regulate the expression of many xenobiotic metabolizing enzymes. Also, the encoded protein can bind specifically to and inhibit the activity of hepatitis B virus. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]