Rabbit Glutamate Receptor, Ionotropic, Delta 2 ELISA Kit | GRID2 elisa kit

Rabbit Glutamate Receptor, Ionotropic, Delta 2 ELISA Kit

Cat. Num. AAA744777

• Gene Names: GRID2; GluD2
• Reactivity: Rabbit
• Synonyms: Glutamate Receptor; Ionotropic; Delta 2; Rabbit Glutamate Receptor; Delta 2 ELISA Kit; GRID2 elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This GRID2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit GRID2. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This GRID2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit GRID2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRID2 elisa kit

Principle of the Assay: GRID2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRID2 antibody and an GRID2-HRP conjugate. The assay sample and buffer are incubated together with GRID2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRID2 concentration since GRID2 from samples and GRID2-HRP conjugate compete for the anti-GRID2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GRID2 from the sample, fewer sites are left to bind GRID2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRID2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GRID2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 157384977
• NCBI GeneID: 2895
• NCBI Accession #: NP_001501.2
• NCBI GenBank Nucleotide #: NM_001510.3
• Molecular Weight: 102,822 Da
• NCBI Official Full Name: glutamate receptor ionotropic, delta-2 isoform 1
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, delta 2
• NCBI Official Symbol: GRID2
• NCBI Official Synonym Symbols: GluD2
• NCBI Protein Information: glutamate receptor ionotropic, delta-2; gluR delta-2 subunit; glutamate receptor delta-2 subunit
• UniProt Protein Name: Glutamate receptor ionotropic, delta-2
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: GRID2
• UniProt Synonym Gene Names: GLURD2; GluD2; GluR delta-2 subunit
• UniProt Entry Name: GRID2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. The encoded protein is a multi-pass membrane protein that is expressed selectively in cerebellar Purkinje cells. A point mutation in the mouse ortholog, associated with the phenotype named 'lurcher', in the heterozygous state leads to ataxia resulting from selective, cell-autonomous apoptosis of cerebellar Purkinje cells during postnatal development. Mice homozygous for this mutation die shortly after birth from massive loss of mid- and hindbrain neurons during late embryogenesis. This protein also plays a role in synapse organization between parallel fibers and Purkinje cells. Alternate splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause cerebellar ataxia in humans. [provided by RefSeq, Apr 2014]

Uniprot Description

GluR-delta2: Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRID2 subfamily.

Protein type: Channel, calcium; Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4q22

Cellular Component: postsynaptic membrane; integral to plasma membrane; dendrite; plasma membrane; dendritic spine; ionotropic glutamate receptor complex; synapse; cell junction

Molecular Function: extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; glutamate receptor activity; PDZ domain binding

Biological Process: heterophilic cell adhesion; synaptic transmission, glutamatergic; prepulse inhibition; transport; ionotropic glutamate receptor signaling pathway; glutamate signaling pathway; regulation of neuron apoptosis; regulation of excitatory postsynaptic membrane potential; cerebellar granule cell differentiation

Disease: Spinocerebellar Ataxia, Autosomal Recessive 18