Mouse Glutamate Receptor, Ionotropic, Delta 2 Interacting Protein ELISA Kit | GRId2IP elisa kit

Mouse Glutamate Receptor, Ionotropic, Delta 2 Interacting Protein ELISA Kit

Cat. Num. AAA744319

• Reactivity: Mouse
• Synonyms: Glutamate Receptor; Ionotropic; Delta 2 Interacting Protein; Mouse Glutamate Receptor; Delta 2 Interacting Protein ELISA Kit; GRId2IP elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This GRID2IP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GRID2IP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRId2IP elisa kit

Principle of the Assay: GRID2IP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRID2IP antibody and an GRID2IP-HRP conjugate. The assay sample and buffer are incubated together with GRID2IP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRID2IP concentration since GRID2IP from samples and GRID2IP-HRP conjugate compete for the anti-GRID2IP antibody binding site. Since the number of sites is limited, as more sites are occupied by GRID2IP from the sample, fewer sites are left to bind GRID2IP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRID2IP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GRId2IP elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 49523342
• NCBI GeneID: 170935
• Molecular Weight: 113,535 Da
• NCBI Official Full Name: Grid2ip protein
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, delta 2 (Grid2) interacting protein 1
• NCBI Official Symbol: Grid2ip
• NCBI Protein Information: delphilin; L-delphilin; GluR-delta2 philic-protein; glutamate receptor, ionotropic, delta 2-interacting protein 1
• UniProt Protein Name: Delphilin
• Protein Family: Delphilin
• UniProt Gene Name: Grid2ip
• UniProt Entry Name: GRD2I_MOUSE

Uniprot Description

GRID2IP: Postsynaptic scaffolding protein at the parallel fiber- Purkinje cell synapse, where it may serve to link GRID2 with actin cytoskeleton and various signaling molecules.

Protein type: Adaptor/scaffold

Cellular Component: postsynaptic membrane; cell projection; membrane; plasma membrane; dendritic spine; synapse; cell junction

Molecular Function: protein binding

Biological Process: metabotropic glutamate receptor signaling pathway; actin cytoskeleton organization and biogenesis