Testing Data
General Alkaline Phosphatase Staining Kit
Alkaline Phosphatase Staining Kit
Reactivity
General
Synonyms
Alkaline Phosphatase; Alkaline Phosphatase Staining Kit; Alkaline Phosphatase Kit; AP Staining; Phosphatase; AP Staining Kit; Alkaline Phosphatase Staining; Alkaline Phosphatase staining kit
Reactivity
General
Samples
Stem cells
Induced pluripotent stem cells
Cell lines containing Alkaline Phosphataseactivity
Induced pluripotent stem cells
Cell lines containing Alkaline Phosphataseactivity
Features & Benefits
AP Staining in stem cells/pluripotent stem cells or stem cells undergoing osteogenesis.
Staining followed by microscopy
Screening for pluripotent cells induced by various factors.
Detection of alkaline phosphate activity in stem cells/pluripotent stem cells, HeLa, HepG2, HEK293 cells, osteoblasts and osteocytes.
To identify and screen Alkaline Phosphatase activity in cells, Rapid, Cost-effective, Reliable
Simple
Staining followed by microscopy
Screening for pluripotent cells induced by various factors.
Detection of alkaline phosphate activity in stem cells/pluripotent stem cells, HeLa, HepG2, HEK293 cells, osteoblasts and osteocytes.
To identify and screen Alkaline Phosphatase activity in cells, Rapid, Cost-effective, Reliable
Simple
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Related Product Information for Alkaline Phosphatase staining kit
The Alkaline Phosphatase (AP) Staining Kit provides a quick and easy method to stain Alkaline Phosphatase in pluripotent stem cells. It is based on the ability of Alkaline Phosphatase to hydrolyze the phosphate group on the substrate, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) to form a blue colored intermediate. Nitro Blue Tetrazolium (NBT) then oxidizes the intermediate to form an insoluble, dark blue dimer. Because stem cells/pluripotent stem cells have increased AP activity, the AP Staining Kit can be used to identify and screen Alkaline Phosphatase activity in these cells. The staining method is simple, rapid, cost-effective and reliable. The entire procedure can be performed in the same microtiter plate and does not require any extra steps such as fixing or harvesting the cells. The assay can be also used as the initial step to test the pluripotency of cells and to monitor osteogenic differentiation of pluripotent/stem cells.
Product Categories/Family for Alkaline Phosphatase staining kit
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Product Notes
The General Alkaline Phosphatase (Catalog #AAA849608) is a Staining Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849608 Staining Kit recognizes General Alkaline Phosphatase. It is sometimes possible for the material contained within the vial of "Alkaline Phosphatase, Staining Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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