Typical Testing Data/Standard Curve (for reference only)
General cAMP ELISA Kit | cAMP elisa kit
cAMP (Cyclic adenosine monophosphate) ELISA Kit
Gene Names
CHAMP1; CAMP; CHAMP; ZNF828; C13orf8
Reactivity
General
Synonyms
cAMP; cAMP (Cyclic adenosine monophosphate) ELISA Kit; cAMP elisa kit
Reactivity
General
Specificity
This kit recognizes Bovine, Horse, Sheep E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.
Sequence Length
812
Samples
Serum And Plasma Samples
Assay Type
Quantitative Competitive
Detection Range
9.37-600pg/mL
Sensitivity
5.63pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level E2 were tested 20 times on one plate, respectively
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level E2 were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for cAMP elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Bovine, Horse, Sheep E2 concentrations in serum and plasma samples. Please contact tech-support for other sample type detection.
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E2. During the reaction, E2 in the sample or standard competes with a fixed amount of E2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to E2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve!!Background/Introduction: Estradiol is a female sex hormone produced by the ovaries, adrenal gland and also the placenta during pregnancy. Estradiol is the most important hormone during a female's reproductive years, and is required for reproductive and sexual function as well as having an impact on the health of other organs and tissues. In females, estradiol acts primarily as a growth hormone for the reproductive organs including the vagina, the fallopian tubes, the endometrium and the cervical glands [1]. Estradiol also enhances growth of the womb's muscle layer, the myometrium. In addition, the hormone maintains oocytes and triggers a series of events that lead to ovulation. Though estradiol levels in men are much lower compared to those in women, estradiol has important roles in men as well. Aside from humans and other mammals, estradiol is also found in most vertebrates and crustaceans, insects, fish, and other animal species. Estradiol is produced especially within the follicles of the ovaries, but also in other tissues including the testicles, the adrenal glands, fat, liver, the breasts, and the brain. Estradiol is produced in the body from cholesterol through a series of reactions and intermediates [2].
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E2. During the reaction, E2 in the sample or standard competes with a fixed amount of E2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to E2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve!!Background/Introduction: Estradiol is a female sex hormone produced by the ovaries, adrenal gland and also the placenta during pregnancy. Estradiol is the most important hormone during a female's reproductive years, and is required for reproductive and sexual function as well as having an impact on the health of other organs and tissues. In females, estradiol acts primarily as a growth hormone for the reproductive organs including the vagina, the fallopian tubes, the endometrium and the cervical glands [1]. Estradiol also enhances growth of the womb's muscle layer, the myometrium. In addition, the hormone maintains oocytes and triggers a series of events that lead to ovulation. Though estradiol levels in men are much lower compared to those in women, estradiol has important roles in men as well. Aside from humans and other mammals, estradiol is also found in most vertebrates and crustaceans, insects, fish, and other animal species. Estradiol is produced especially within the follicles of the ovaries, but also in other tissues including the testicles, the adrenal glands, fat, liver, the breasts, and the brain. Estradiol is produced in the body from cholesterol through a series of reactions and intermediates [2].
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89,099 Da
NCBI Official Full Name
chromosome alignment-maintaining phosphoprotein 1
NCBI Official Synonym Full Names
chromosome alignment maintaining phosphoprotein 1
NCBI Official Symbol
CHAMP1
NCBI Official Synonym Symbols
CAMP; CHAMP; ZNF828; C13orf8
NCBI Protein Information
chromosome alignment-maintaining phosphoprotein 1; zinc finger protein 828
UniProt Protein Name
Chromosome alignment-maintaining phosphoprotein 1
Protein Family
UniProt Gene Name
CHAMP1
UniProt Synonym Gene Names
C13orf8; CAMP; KIAA1802; ZNF828
UniProt Entry Name
CHAP1_HUMAN
Uniprot Description
ZNF828: Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.
Protein type: C2H2-type zinc finger protein; RNA-binding
Chromosomal Location of Human Ortholog: 13q34
Cellular Component: nucleoplasm; cytoplasm; spindle; condensed chromosome; nucleus
Molecular Function: protein binding; nucleic acid binding; metal ion binding
Biological Process: attachment of spindle microtubules to kinetochore during mitosis; sister chromatid biorientation
Research Articles on cAMP
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Product Notes
The General cAMP champ1 (Catalog #AAA2511445) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2511445 ELISA Kit recognizes General cAMP. It is sometimes possible for the material contained within the vial of "cAMP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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