Rabbit Zap70 Polyclonal Antibody | anti-Zap70 antibody

Zap70 Antibody (Tyr319)

Cat. Num. AAA5313598

• Gene Names: ZAP70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
• Applications: Western Blot
• Purity: Affinity Chromatography
• Synonyms: Zap70; Polyclonal Antibody; Zap70 Antibody (Tyr319); Rabbit Polyclonal Zap70 Antibody (Tyr319); Polyclonal Zap70 antibody; Anti-Zap70 antibody; 70 kDa zeta-associated protein antibody; FLJ17670 antibody; FLJ17679 antibody; SRK antibody; STD antibody; Syk-related tyrosine kinase antibody; Tyrosine-protein kinase ZAP-70 antibody; TZK antibody; ZAP-70 antibody; ZAP70 antibody; zeta-chain (TCR) associated protein kinase 70kDa antibody; zeta-chain associated protein kinase 70kD antibody; anti-Zap70 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Zap70 antibody (Tyr319) detects endogenous levels of Zap70 only when phosphorylated at tyrosine 319
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 493

• Applicable Applications for anti-Zap70 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Zap70 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human Zap70 around the phosphorylation site of tyrosine 319 (S-P-YP-S-D) as the immunogen.
• Cross-Reactivity: Mouse, Rat
• Preparation and Storage: Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extract from Jurkat cells, using Zap-70 antibody and Zap70 antibody (Tyr319) (Lanes 3 and 4))

Related Product Information for anti-Zap70 antibody

Zap70 is a protein normally expressed near the surface membrane of T cells and natural killer cells. It is part of the T cell receptor, and plays a critical role in T-cell signaling.

Product Categories/Family for anti-Zap70 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 24657846
• NCBI GeneID: 7535
• NCBI Official Full Name: ZAP70 protein
• NCBI Official Synonym Full Names: zeta chain of T cell receptor associated protein kinase 70
• NCBI Official Symbol: ZAP70
• NCBI Official Synonym Symbols: SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
• NCBI Protein Information: tyrosine-protein kinase ZAP-70
• Protein Family: Tyrosine-protein kinase

NCBI Description

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]