Typical Testing Data/Standard Curve (for reference only)
cAMP assay kit
cAMP Direct Immunoassay Kit (Colorimetric)
Gene Names
CHAMP1; CAMP; CHAMP; ZNF828; C13orf8
Synonyms
cAMP; cAMP Direct Immunoassay Kit (Colorimetric); cAMP assay kit
Sequence Length
812
Samples
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Features & Benefits
Simple procedure
Fast and convenient
The assay is sensitive, stable and high-throughput adaptable
The kit provides a new acetylation procedure to improve detection sensitivity significantly
Fast and convenient
The assay is sensitive, stable and high-throughput adaptable
The kit provides a new acetylation procedure to improve detection sensitivity significantly
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for cAMP assay kit
Product Information: cAMP Direct Immunoassay Kit: Direct Competitive Immunoassay for Sensitive and Quantitative Determination of cAMP level in Biological Samples. Detection Limit: ~ 0.02 - 2 muM. 100 Assays.
Background: Adenosine 3',5'-cyclic monophosphate (cyclic AMP, cAMP) is an important "second messenger" involved in many physiological processes. MyBioSource's cAMP Assay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cAMP level in biological samples. The kit utilizes recombinant Protein G coated 96-well plate to efficiently anchor cAMP polyclonal antibody on to the plate. cAMP-HRP conjugate directly competes with cAMP from sample binding to the cAMP antibody on the plate. After incubation and washing, the amount of cAMP-HRP bound to the plate can easily be determined by reading HRP activity at OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cAMP in samples. In addition, the kit provides a new acetylation procedure to improve detection sensitivity significantly. The kit can detect ~0.1-10 pmol/5 mul (or ~ 0.02-2 muM) cAMP samples.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Applications- The kit can detect ~0.1-10 pmol/5 mul (or ~ 0.02-2 muM) cAMP samples.
Background: Adenosine 3',5'-cyclic monophosphate (cyclic AMP, cAMP) is an important "second messenger" involved in many physiological processes. MyBioSource's cAMP Assay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cAMP level in biological samples. The kit utilizes recombinant Protein G coated 96-well plate to efficiently anchor cAMP polyclonal antibody on to the plate. cAMP-HRP conjugate directly competes with cAMP from sample binding to the cAMP antibody on the plate. After incubation and washing, the amount of cAMP-HRP bound to the plate can easily be determined by reading HRP activity at OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cAMP in samples. In addition, the kit provides a new acetylation procedure to improve detection sensitivity significantly. The kit can detect ~0.1-10 pmol/5 mul (or ~ 0.02-2 muM) cAMP samples.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Applications- The kit can detect ~0.1-10 pmol/5 mul (or ~ 0.02-2 muM) cAMP samples.
Product Categories/Family for cAMP assay kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89,099 Da
NCBI Official Full Name
chromosome alignment-maintaining phosphoprotein 1
NCBI Official Synonym Full Names
chromosome alignment maintaining phosphoprotein 1
NCBI Official Symbol
CHAMP1
NCBI Official Synonym Symbols
CAMP; CHAMP; ZNF828; C13orf8
NCBI Protein Information
chromosome alignment-maintaining phosphoprotein 1
UniProt Protein Name
Chromosome alignment-maintaining phosphoprotein 1
Protein Family
UniProt Gene Name
CHAMP1
UniProt Synonym Gene Names
C13orf8; CAMP; CHAMP; KIAA1802; ZNF828
UniProt Entry Name
CHAP1_HUMAN
Uniprot Description
ZNF828: Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.
Protein type: C2H2-type zinc finger protein; RNA-binding
Chromosomal Location of Human Ortholog: 13q34
Cellular Component: nucleoplasm; cytoplasm; condensed chromosome; spindle; nucleus
Molecular Function: protein binding; nucleic acid binding; metal ion binding
Biological Process: sister chromatid biorientation; attachment of spindle microtubules to kinetochore during mitosis
Research Articles on cAMP
Similar Products
Product Notes
The cAMP champ1 (Catalog #AAA841511) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "cAMP, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
