Typical Testing Data/Standard Curve (for reference only)
Beta-Hydroxybutyrate Assay Kit
Beta-Hydroxybutyrate (Ketone Body) Assay Kit (Colorimetric)
Synonyms
Beta-Hydroxybutyrate; Beta-Hydroxybutyrate (Ketone Body) Assay Kit (Colorimetric); Beta-Hydroxybutyrate assay kit
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store at -80 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Beta-Hydroxybutyrate assay kit
Principle of the Assay: beta-Hydroxybutyrate Assay Kit is a convenient quantitative tool that measures beta-Hydroxybutyrate within biological samples. The assay is based on an enzymatic cycling reaction in which the cofactor NAD+ is reduced to NADH. NADH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm. The intensity of the product color is proportional to the beta-Hydroxybutyrate within a sample. Samples and standards are incubated for 30 minutes and then read with a standard 96-well colorimetric plate reader (Figure 1). Samples are compared to a known concentration of beta-Hydroxybutyrate standards within the 96-well microtiter plate format.
Background: Ketone bodies are water-soluble molecules produced from fatty acid oxidation in the liver and kidney. Acetoacetate, beta-hydroxybutyrate (beta-HB), and their decarboxylated degradation product, acetone, are the three primary ketone bodies. beta-HB is the reduced form of acetoacetate in which a ketone group is converted to an alcohol. beta-HB and acetoacetate can be used as an energy source when glucose stores are depleted. Ketone bodies are produced under physiological conditions as fasting, starvation, prolonged and intense exercise, low carbohydrate diets, or untreated type 1 diabetes mellitus. During these instances, gluconeogenesis in the liver produces acetoacetate via two acetyl CoA molecules. beta-HB and acetoacetate are transported to peripheral tissues and reconverted into acetyl-CoA for energy via their cells' citric acid cycles. Acetone must be converted in the liver to lactic acid and then pyruvic acid before being converted to acetyl-CoA. It is usually excreted in the urine or exhaled. High levels of ketone bodies can lead to ketosis, or as in extreme type 1 diabetes, ketoacidosis. Prolonged ketosis may lead to a life threatening metabolic acidosis. Pathological ketosis may indicate organ failure, hypoglycemia in children, diabetes, alcohol intoxication, corticosteroid or growth hormone insufficiency. The degree of ketosis can be determined by monitoring the blood levels of beta-HB. Ketone bodies are noted for their sweet smell and can be detected in the breath and urine of people with ketosis or ketoacidosis. beta-Hydroxybutyrate is the ketoacid most prevalent in serum and accounts for ~75% of all ketone bodies measured. During ketosis, beta-HB increases even more than the other ketone bodies and is a more accurate index of ketoacidosis. beta-Hydroxybutyrate Assay Kit is a simple colorimetric assay that measures beta-Hydroxybutyrate present in biological samples such as plasma, serum, urine, cell lysates or tissue extracts in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, beta-Hydroxybutyrate standards and unknown samples. The total beta-Hydroxybutyrate concentrations of unknown samples are determined by comparison with a known beta-Hydroxybutyrate standard. The kit has a detection sensitivity limit of approximately ~30 uM beta-Hydroxybutyrate.
Background: Ketone bodies are water-soluble molecules produced from fatty acid oxidation in the liver and kidney. Acetoacetate, beta-hydroxybutyrate (beta-HB), and their decarboxylated degradation product, acetone, are the three primary ketone bodies. beta-HB is the reduced form of acetoacetate in which a ketone group is converted to an alcohol. beta-HB and acetoacetate can be used as an energy source when glucose stores are depleted. Ketone bodies are produced under physiological conditions as fasting, starvation, prolonged and intense exercise, low carbohydrate diets, or untreated type 1 diabetes mellitus. During these instances, gluconeogenesis in the liver produces acetoacetate via two acetyl CoA molecules. beta-HB and acetoacetate are transported to peripheral tissues and reconverted into acetyl-CoA for energy via their cells' citric acid cycles. Acetone must be converted in the liver to lactic acid and then pyruvic acid before being converted to acetyl-CoA. It is usually excreted in the urine or exhaled. High levels of ketone bodies can lead to ketosis, or as in extreme type 1 diabetes, ketoacidosis. Prolonged ketosis may lead to a life threatening metabolic acidosis. Pathological ketosis may indicate organ failure, hypoglycemia in children, diabetes, alcohol intoxication, corticosteroid or growth hormone insufficiency. The degree of ketosis can be determined by monitoring the blood levels of beta-HB. Ketone bodies are noted for their sweet smell and can be detected in the breath and urine of people with ketosis or ketoacidosis. beta-Hydroxybutyrate is the ketoacid most prevalent in serum and accounts for ~75% of all ketone bodies measured. During ketosis, beta-HB increases even more than the other ketone bodies and is a more accurate index of ketoacidosis. beta-Hydroxybutyrate Assay Kit is a simple colorimetric assay that measures beta-Hydroxybutyrate present in biological samples such as plasma, serum, urine, cell lysates or tissue extracts in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, beta-Hydroxybutyrate standards and unknown samples. The total beta-Hydroxybutyrate concentrations of unknown samples are determined by comparison with a known beta-Hydroxybutyrate standard. The kit has a detection sensitivity limit of approximately ~30 uM beta-Hydroxybutyrate.
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Product Notes
The Beta-Hydroxybutyrate (Catalog #AAA169325) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Beta-Hydroxybutyrate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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