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Typical Testing Data/Standard Curve (for reference only) ()

General Chitinase Assay Kit

Chitinase Microplate Assay Kit

Reactivity
General
Applications
Functional Assay
Synonyms
Chitinase; Chitinase Microplate Assay Kit; EC 3.2.1.14; 1; 4-beta-poly-N-acetylglucosaminidase; Chitodextrinase; Poly-beta-glucosaminidase; Chitinase assay kit
Ordering
For Research Use Only!
Reactivity
General
Applicable Applications for Chitinase assay kit
Functional Assay
Samples
Tissue extracts, cell lysate, cell culture media and other biological fluids samples
Detection Range
0.01 mg/ml - 1 mg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

()

Typical Testing Data/Standard Curve (for reference only) ()
Related Product Information for Chitinase assay kit
Background/Introduction: Chitinases (EC 3.2.1.14) are hydrolytic enzymes that break down glycosidic bonds in chitin. Chitinase catalyzes the hydrolytic cleavage of the beta-1?4-glycoside bond present in biopolymers of N-acetylglucosamine, primarily in chitin. Chitinases are widely distributed in living organisms and are found in fungi, bacteria, parasites, plants, and animals. They are classified in families based on amino acid sequence similarities. As chitin is a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods), chitinases are generally found in organisms that either need to reshape their own chitin or dissolve and digest the chitin of fungi or animals. Chitinases perform different functions in different organisms. In bacteria, they are mainly involved in nutritional processes. In yeast and various fungi, these enzymes participate in morphogenesis. In animals and plants, chitinases primarily play a role in the defense of the organism against pathogen attack. The assay is initiated with the enzymatic hydrolysis of the chitin by chitinases. The enzyme catalysed reaction products N-acetylglucosamine react with PDAB, and can be measured at a colorimetric readout at 585 nm.

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Product Notes

The General Chitinase (Catalog #AAA8243204) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8243204 Assay Kit recognizes General Chitinase. AAA Biotech's Chitinase can be used in a range of immunoassay formats including, but not limited to, Functional Assay. Researchers should empirically determine the suitability of the Chitinase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chitinase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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