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Typical Testing Data/Standard Curve (for reference only)

General Glycolate Oxidase Assay Kit | HAO1 assay kit

Glycolate Oxidase Microplate Assay Kit

Gene Names
HAO1; GOX; GOX1; HAOX1
Reactivity
General
Applications
Functional Assay
Synonyms
Glycolate Oxidase; Glycolate Oxidase Microplate Assay Kit; EC 1.1.3.15; (S)-2-hydroxy-acid oxidase; Glycolate oxidase; Hydroxy-acid oxidase A; Hydroxy-acid oxidase B; HAO1; GOX; GOX1; HAOX1; HAO1 assay kit
Ordering
For Research Use Only!
Reactivity
General
Sequence Length
370
Applicable Applications for HAO1 assay kit
Functional Assay
Samples
Tissue extracts, cell lysate, cell culture media and other biological fluids samples
Detection Range
0.05 mmol/L - 5 mmol/L
Preparation and Storage
Shipped and store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HAO1 assay kit
Background/Introduction: Glycolate oxidase is a member of the superfamily of the a-hydroxy acid oxidases (HAO), enzymes that are present in both plants and animals. It catalyzes the FMN-mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. The assay is initiated with the enzymatic oxidization of the Glycolic acid by Glycolate oxidase. The enzyme catalysed reaction product Glyoxylic acid react with Phenylhydrazine, glyoxylate phenylhydrazone can be measured at a colorimetric readout at 500 nm.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
40,924 Da
NCBI Official Full Name
glycolate oxidase
NCBI Official Synonym Full Names
hydroxyacid oxidase 1
NCBI Official Symbol
HAO1
NCBI Official Synonym Symbols
GOX; GOX1; HAOX1
NCBI Protein Information
hydroxyacid oxidase 1
UniProt Protein Name
Hydroxyacid oxidase 1
Protein Family
UniProt Gene Name
HAO1
UniProt Synonym Gene Names
GOX1; HAOX1; HAOX1; GOX
UniProt Entry Name
HAOX1_HUMAN

NCBI Description

This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008]

Uniprot Description

HAO1: Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2- hydroxy octanoate. Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.

Protein type: Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 1.1.3.15; Oxidoreductase

Chromosomal Location of Human Ortholog: 20p12

Cellular Component: peroxisomal matrix; peroxisome

Molecular Function: (S)-2-hydroxy-acid oxidase activity; FMN binding; glycolate oxidase activity; glyoxylate oxidase activity; receptor binding

Biological Process: fatty acid alpha-oxidation; glycolate catabolic process; glyoxylate metabolic process; response to oxidative stress

Research Articles on HAO1

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Product Notes

The General HAO1 hao1 (Catalog #AAA8243218) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8243218 Assay Kit recognizes General HAO1. AAA Biotech's Glycolate Oxidase can be used in a range of immunoassay formats including, but not limited to, Functional Assay. Researchers should empirically determine the suitability of the HAO1 hao1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glycolate Oxidase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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