Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidise conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma)
Rabbit GCK Polyclonal Antibody | anti-GCK antibody
GCK Antibody
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Affinity Chromatography
Synonyms
GCK; Polyclonal Antibody; GCK Antibody; Rabbit Polyclonal GCK Antibody; Polyclonal GCK antibody; anti-GCK antibody; Hexokinase D antibody; HK IV antibody; Glucokinase antibody; Hexokinase type IV antibody; HK4 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer with 0.09% (W/V) Sodium Azide
Concentration
250 ug/ml (varies by lot)
Applicable Applications for anti-GCK antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ELISA: 0.25 ug/ml
IHC: 5-25 ug/ml
WB: 0.5-2.5 ug/ml
IHC: 5-25 ug/ml
WB: 0.5-2.5 ug/ml
Immunogen
GCK antibody was raised in Rabbit using synthetic peptide selected from the N-terminal region of human GCK as the immunogen.
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidise conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidise conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma)
Western Blot (WB)
(Western blot analysis of antibodyto detect GCK in HepG2 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).)
Western Blot (WB)
(Western blot analysis of antibodyto detect GCK in HepG2 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).)
Related Product Information for anti-GCK antibody
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of the gene for GCK results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in the gene have been associated with non-insulin dependent diabetes mellitus, also called maturity onset diabetes of the young, type 2 ; mutations have also been associated with persistent hyperinsulinemic hypoglycemia of infancy.
Product Categories/Family for anti-GCK antibody
Similar Products
Product Notes
The GCK (Catalog #AAA5311193) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GCK can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 0.25 ug/ml IHC: 5-25 ug/ml WB: 0.5-2.5 ug/ml. Researchers should empirically determine the suitability of the GCK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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