Typical Testing Data/Standard Curve (for reference only)
(Mouse GDF-15 PicoKine ELISA Kit standard curve )
Mouse GDF-15 ELISA Kit | GDF15 elisa kit
Mouse GDF-15 PicoKine ELISA Kit
Gene Names
Gdf15; SBF; MIC-1; NAG-1
Reactivity
Mouse
Synonyms
GDF-15; Mouse GDF-15 PicoKine ELISA Kit; Growth/differentiation factor 15; growth differentiation factor 15; Macrophage inhibitory cytokine 1; MIC 1; NAG-1; NRG-1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PDF; PLAB; Placental bone morphogenetic protein; Placental TGF-beta; Prostate differentiation factor; PROSTATE-DERIVED FACTOR; PTGFB; GDF15 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse GDF-15
Sequence Length
303
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse GDF-15 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse GDF-15 PicoKine ELISA Kit standard curve )
Related Product Information for GDF15 elisa kit
Principle of the assay: mouse GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for GDF-15 has been precoated onto 96-well plates. Standards(NSO, S189-A303) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse GDF-15 amount of sample captured in plate.
Background: GDF-15(Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.
Background: GDF-15(Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
33,225 Da
NCBI Official Full Name
Growth/differentiation factor 15
NCBI Official Synonym Full Names
growth differentiation factor 15
NCBI Official Symbol
Gdf15
NCBI Official Synonym Symbols
SBF; MIC-1; NAG-1
NCBI Protein Information
growth/differentiation factor 15; GDF-15; macrophage inhibiting cytokine-1
UniProt Protein Name
Growth/differentiation factor 15
Protein Family
UniProt Gene Name
Gdf15
UniProt Synonym Gene Names
Sbf; GDF-15
UniProt Entry Name
GDF15_MOUSE
Uniprot Description
GDF15: disulfide-linked. Belongs to the TGF-beta family.
Protein type: Secreted, signal peptide; Secreted; Cytokine
Cellular Component: extracellular space; extracellular region
Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding
Biological Process: regulation of apoptosis; regulation of MAPKKK cascade; cell development
Research Articles on GDF15
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Product Notes
The Mouse GDF15 gdf15 (Catalog #AAA177350) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177350 ELISA Kit recognizes Mouse GDF15. It is sometimes possible for the material contained within the vial of "GDF-15, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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