Frizzled 5 Recombinant Protein | FZD5 recombinant protein

Frizzled 5 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122979

• Gene Names: Fzd5; Fz5; Fz-5; mFz5; AI427138; 5330434N09Rik
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Frizzled 5; Frizzled 5 Protein; Mouse; Recombinant (hFc Tag); Mouse Frizzled-5/FZD5 Protein (Fc Tag); 5330434N09Rik Protein; AI427138 Protein; Fz-5 Protein; Fz5 Protein; mFz5 Protein; frizzled class receptor 5; FZD5 recombinant protein

• Host: CHO Stable Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro167

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 27
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse FZD5 (Q9EQD0)(Met1-Pro167) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for FZD5 recombinant protein

Background: Wnt signaling is involved in a variety of embryonic development processes of nonvertebrates and vertebrates, where it determines cell motility, cell polarity, differentiation, proliferation and apoptosis, as well as formation of neural synapses. Various homologs of the Wingless protein, termed WNT factors, represent key mediators and act through a receptor complex comprised of members of the Frizzled and low density lipoprotein-related receptors (LRP). 19 WNTs, 1 Frizzled, and 2 LRP proteins have been identified. Frizzled is a family of G protein-coupled receptor proteins consisting of a divergent signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail.Frizzled 5 (FZD5) is believed to be the receptor for the Wnt5A ligand, and also interactions with Wnt1B, Wnt2B, and Wnt 7A functionally. Recent studies of WNT5A/Frizzled-5 signaling have revealed an unexpected and novel role in orchestrating adaptive immunity in response to microbial stimulation. In addition, FZD5 is also implicated in the survival of mature neurons in the parafascicular nucleus of the thalamus.

Product Categories/Family for FZD5 recombinant protein

Non-Canonical Wnt Pathway

References

Wang Y., et al.,(1996), A large family of putative transmembrane receptors homologous to the product of the Drosophila tissue polarity gene frizzled. J. Biol. Chem. 271:4468-4476.Saitoh T., et al., (2001), Molecular cloning and characterization of human Frizzled-5 gene on chromosome 2q33.3-q34 region.Int. J. Oncol. 19:105-110.Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.

NCBI and Uniprot Product Information

• NCBI GI #: 111160873
• NCBI GeneID: 14367
• NCBI Accession #: NP_001036124.1
• NCBI GenBank Nucleotide #: NM_001042659.1
• UniProt Accession #: Q9EQD0; O08975; Q8BMR2; Q8CHK9; G5E8F0
• Molecular Weight: 64,138 Da
• NCBI Official Full Name: frizzled-5
• NCBI Official Synonym Full Names: frizzled homolog 5 (Drosophila)
• NCBI Official Symbol: Fzd5
• NCBI Official Synonym Symbols: Fz5; Fz-5; mFz5; AI427138; 5330434N09Rik
• NCBI Protein Information: frizzled-5
• UniProt Protein Name: Frizzled-5
• Protein Family: Frizzled
• UniProt Gene Name: Fzd5
• UniProt Synonym Gene Names: Fz-5; mFz5
• UniProt Entry Name: FZD5_MOUSE

Uniprot Description

FZD5: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta- catenin pathway. Interacts with GOPC. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, Fz/Smo family; Receptor, GPCR

Cellular Component: Golgi apparatus; cell surface; tight junction; membrane; perinuclear region of cytoplasm; early endosome; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; signal transducer activity; protein binding; Wnt receptor activity; transmembrane receptor activity; ubiquitin protein ligase binding; protein kinase binding

Biological Process: positive regulation of JNK activity; post-embryonic camera-type eye development; Wnt receptor signaling pathway; multicellular organismal development; embryonic axis specification; cell maturation; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; signal transduction; embryonic camera-type eye development; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; positive regulation of interferon-gamma production; cell surface receptor linked signal transduction; vasculature development; determination of anterior/posterior axis, embryo; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; cell differentiation; Spemann organizer formation