Frizzled-5 / FZD5 Recombinant Protein | FZD5 recombinant protein

Recombinant Human Frizzled-5 / FZD5 Protein (His & Fc tag)

Cat. Num. AAA2546237

• Gene Names: FZD5; HFZ5; C2orf31
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: Frizzled-5 / FZD5; Recombinant Human Frizzled-5 / FZD5 Protein (His & Fc tag); FZD5; C2orf31; DKFZp434E2135; HFZ5; MGC129692; FZD5 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 585

• Application Notes: The recombinant human frizzled 5/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 389 amino acids and has a predicted molecular mass of 44 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhfrizzled 5/Fc monomer is approximately 58 kDa due to glycosylation.
• Predicted N Terminal: Ala 27
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for FZD5 recombinant protein

Background: Wnt signaling is involved in a variety of embryonic development processes of nonvertebrates and vertebrates, where it determines cell motility, cell polarity, differentiation, proliferation and apoptosis, as well as formation of neural synapses. Various homologs of the Wingless protein, termed WNT factors, represent key mediators and act through a receptor complex comprised of members of the Frizzled and low density lipoprotein-related receptors (LRP). 19 WNTs, 10 Frizzled, and 2 LRP proteins have been identified. Frizzled is a family of G protein-coupled receptor proteins consisting of a divergent signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail.Frizzled 5 (FZD5) is believed to be the receptor for the Wnt5A ligand, and also interactions with Wnt10B, Wnt2B, and Wnt 7A functionally. Recent studies of WNT5A/Frizzled-5 signaling have revealed an unexpected and novel role in orchestrating adaptive immunity in response to microbial stimulation. In addition, FZD5 is also implicated in the survival of mature neurons in the parafascicular nucleus of the thalamus.

Description: A DNA sequence encoding the extracellular domain (Met 1-Pro 167) of human frizzled 5 (NP_003459.2) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 27894385
• NCBI GeneID: 7855
• NCBI Accession #: NP_003459.2
• NCBI GenBank Nucleotide #: NM_003468.3
• UniProt Accession #: Q13467; Q53R22; A8K2X1; B2RCZ1
• Molecular Weight: 64,507 Da
• NCBI Official Full Name: frizzled-5
• NCBI Official Synonym Full Names: frizzled class receptor 5
• NCBI Official Symbol: FZD5
• NCBI Official Synonym Symbols: HFZ5; C2orf31
• NCBI Protein Information: frizzled-5
• UniProt Protein Name: Frizzled-5
• Protein Family: Frizzled
• UniProt Gene Name: FZD5
• UniProt Synonym Gene Names: C2orf31; Fz-5; hFz5

NCBI Description

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD5: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta- catenin pathway. Interacts with GOPC. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: GPCR, Fz/Smo family; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 2q33.3

Cellular Component: cell surface; early endosome membrane; integral to membrane; plasma membrane

Molecular Function: beta-amyloid binding; G-protein coupled receptor activity; protein binding; protein kinase binding; ubiquitin protein ligase binding; Wnt receptor activity; Wnt-protein binding

Biological Process: determination of anterior/posterior axis, embryo; embryonic axis specification; neuron differentiation; positive regulation of interferon-gamma production; positive regulation of T cell cytokine production; positive regulation of transcription from RNA polymerase II promoter; Spemann organizer formation; synaptogenesis; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, calcium modulating pathway; Wnt receptor signaling pathway, planar cell polarity pathway