Frizzled 10 Recombinant Protein | FZD10 recombinant protein

Frizzled 10 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8122009

• Gene Names: FZD10; Fz10; FzE7; CD350; FZ-10; hFz10
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Frizzled 10; Frizzled 10 Protein; Human; Recombinant (His Tag); Human Frizzled-10/FZD10 Protein (His Tag); CD350 Protein; FZ-10 Protein; Fz10 Protein; FzE7 Protein; hFz10 Protein; frizzled class receptor 10; FZD10 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly161

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ile 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human FZD10 (NP_009128.1)(Met1-Gly161) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for FZD10 recombinant protein

Background: Frizzled-1, also known as Fz-1, CD35 and FZD1, is a multi-pass membrane protein which belongs to theG-protein coupled receptor Fz/Smo family. Frizzled-1/FZD1 is abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord; very low levels in total brain, frontal lobe, temporal lobe and putamen. It is weakly expressed in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate. Frizzled-1/FZD1 is a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Frizzled-1/FZD1 may also be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Product Categories/Family for FZD10 recombinant protein

Non-Canonical Wnt Pathway

References

Kwon H.S., et al.,(2009), Myocilin is a modulator of Wnt signaling. Mol. Cell. Biol. 29:2139-2154.The MGC Project Team, et al., (2004), The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).Genome Res. 14:2121-2127.Tanaka S., et al.,(1998), A novel frizzled gene identified in human esophageal carcinoma mediates APC/beta-catenin signals.Proc. Natl. Acad. Sci. U.S.A. 95:10164-10169.

NCBI and Uniprot Product Information

• NCBI GI #: 6005762
• NCBI GeneID: 11211
• NCBI Accession #: NP_009128.1
• NCBI GenBank Nucleotide #: NM_007197.3
• UniProt Accession #: Q9ULW2
• Molecular Weight: 65,336 Da
• NCBI Official Full Name: frizzled-10
• NCBI Official Synonym Full Names: frizzled family receptor 10
• NCBI Official Symbol: FZD10
• NCBI Official Synonym Symbols: Fz10; FzE7; CD350; FZ-10; hFz10
• NCBI Protein Information: frizzled-10; frizzled homolog 10; frizzled 10, seven transmembrane spanning receptor
• UniProt Protein Name: Frizzled-10
• Protein Family: Frizzled
• UniProt Gene Name: FZD10
• UniProt Synonym Gene Names: Fz-10; hFz10
• UniProt Entry Name: FZD10_HUMAN

NCBI Description

This gene is a member of the frizzled gene family. Members of this family encode 7-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Using array analysis, expression of this intronless gene is significantly up-regulated in two cases of primary colon cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD10: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, Fz/Smo family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24.33

Cellular Component: cell surface; integral to plasma membrane; cytoplasm

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein binding

Biological Process: neuron differentiation; G-protein coupled receptor protein signaling pathway; positive regulation of JNK activity; regulation of actin cytoskeleton organization and biogenesis; Wnt receptor signaling pathway through beta-catenin