Frizzled 10 Recombinant Protein | FZD10 recombinant protein

Frizzled 10 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122827

• Gene Names: Fzd10; Fz-10
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Frizzled 10; Frizzled 10 Protein; Mouse; Recombinant (His Tag); Mouse Frizzled-10/FZD10 Protein (His Tag); Fz-10 Protein; frizzled class receptor 10; FZD10 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly162

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ile 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the N-terminal segment of mouse FZD10 (NP_780493.1)(Met 1-Gly 162) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for FZD10 recombinant protein

Background: Frizzled-1, also known as Fz-1, CD35 and FZD1, is a multi-pass membrane protein which belongs to theG-protein coupled receptor Fz/Smo family. Frizzled-1/FZD1 is abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord; very low levels in total brain, frontal lobe, temporal lobe and putamen. It is weakly expressed in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate. Frizzled-1/FZD1 is a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Frizzled-1/FZD1 may also be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Product Categories/Family for FZD10 recombinant protein

Non-Canonical Wnt Pathway

References

Kwon H.S., et al.,(2009), Myocilin is a modulator of Wnt signaling. Mol. Cell. Biol. 29:2139-2154.The MGC Project Team, et al., (2004), The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).Genome Res. 14:2121-2127.Tanaka S., et al.,(1998), A novel frizzled gene identified in human esophageal carcinoma mediates APC/beta-catenin signals.Proc. Natl. Acad. Sci. U.S.A. 95:10164-10169.

NCBI and Uniprot Product Information

• NCBI GI #: 28202015
• NCBI GeneID: 93897
• NCBI Accession #: NP_780493.1
• NCBI GenBank Nucleotide #: NM_175284.3
• UniProt Accession #: Q8BKG4
• Molecular Weight: 65,318 Da
• NCBI Official Full Name: frizzled-10
• NCBI Official Synonym Full Names: frizzled homolog 10 (Drosophila)
• NCBI Official Symbol: Fzd10
• NCBI Official Synonym Symbols: Fz-10
• NCBI Protein Information: frizzled-10
• UniProt Protein Name: Frizzled-10
• Protein Family: Frizzled
• UniProt Gene Name: Fzd10
• UniProt Synonym Gene Names: Fz10; Fz-10
• UniProt Entry Name: FZD10_MOUSE