CD1D recombinant protein

CD1D, 20-301aa, Human, His-tag, Baculovirus

Cat. Num. AAA206728

• Gene Names: CD1D; R3; CD1A; R3G1
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: CD1D; 20-301aa; Human; His-tag; Baculovirus; Antigen-presenting glycoprotein CD1d isoform 1; CD1A; R3; R3G1; CD1D recombinant protein

• Host: Baculovirus
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: EVPQRLFPLR CLQISSFANS SWTRTDGLAW LGELQTHSWS NDSDTVRSLK PWSQGTFSDQ QWETLQHIFR VYRSSFTRDV KEFAKMLRLS YPLELQVSAG CEVHPGNASN NFFHVAFQGK DILSFQGTSW EPTQEAPLWV NLAIQVLNQD KWTRETVQWL LNGTCPQFVS GLLESGKSEL KKQVKPKAWL SRGPSPGPGR LLLVCHVSGF YPKPVWVKWM RGEQEQQGTQ PGDILPNADE TWYLRATLDV VAGEAAGLSC RVKHSSLEGQ DIVLYWGGSY TSLEHHHHHH
• Sequence Length: 335

• Applicable Applications for CD1D recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CD1D recombinant protein

CD1D, also known as antigen-presenting glycoprotein CD1d isoform 1, is a transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. CD1d-presented lipid antigens activate a special class of T cells, known as natural killer T (NKT) cells, through the interaction with the T-cell receptor present on NKT membranes. When activated, NKT cells rapidly produce Th1 and Th2 cytokines, typically represented by interferon-gamma and interleukin 4 production. Recombinant human CD1D protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CD1D recombinant protein

Immunology

References

Broxmeyer HE., et al, (2012) Blood. 119:5731-5741.; Huh JY., et al, (2017) Diabetes. 66:835-847.

NCBI and Uniprot Product Information

• NCBI GI #: 4502649
• NCBI GeneID: 912
• NCBI Accession #: NP_001757
• NCBI GenBank Nucleotide #: NM_001766.3
• UniProt Accession #: P15813; Q5W0J3; Q9UMM3; Q9Y5M4; D3DVD5
• Molecular Weight: 32.9kDa (290aa) 40-57KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: antigen-presenting glycoprotein CD1d isoform 1
• NCBI Official Synonym Full Names: CD1d molecule
• NCBI Official Symbol: CD1D
• NCBI Official Synonym Symbols: R3; CD1A; R3G1
• NCBI Protein Information: antigen-presenting glycoprotein CD1d
• UniProt Protein Name: Antigen-presenting glycoprotein CD1d
• Protein Family: Antigen-presenting glycoprotein
• UniProt Gene Name: CD1D

NCBI Description

This gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Uniprot Description

Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.