Rabbit KL Polyclonal Antibody | anti-KL antibody

KL Antibody

Cat. Num. AAA9416256

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antigen Affinity Purification
• Synonyms: KL; Polyclonal Antibody; KL Antibody; KLOT; Klotho; Klotho peptide; anti-KL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total KL protein.
• Purity/Purification: Antigen Affinity Purification
• Form/Format: Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Concentration: 0.9 mg/ml (varies by lot)
• Sequence Length: 1012

• Applicable Applications for anti-KL antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:200-1:1000
• Immunogen: Synthetic peptide of human KL
• Immunogen Type: Peptide
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Gel: 10%SDS-PAGE Lysate: 40 μ g Lane: Jurkat cells Primary antibody: 1/250 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 2 minutes)

Related Product Information for anti-KL antibody

This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss.

Product Categories/Family for anti-KL antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 24497614
• NCBI GeneID: 9365
• NCBI Accession #: NP_004786.2
• NCBI GenBank Nucleotide #: NM_004795.3
• UniProt Accession #: Q9UEF7; Q5VZ95; Q96KV5; Q96KW5; Q9UEI9; Q9Y4F0
• Molecular Weight: 62,135 Da
• NCBI Official Full Name: klotho
• NCBI Official Synonym Full Names: klotho
• NCBI Official Symbol: KL
• NCBI Protein Information: klotho
• UniProt Protein Name: Klotho
• Protein Family: Klotho
• UniProt Gene Name: KL
• UniProt Entry Name: KLOT_HUMAN

Uniprot Description

Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.1.31; Hydrolase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: extracellular region; integral to plasma membrane; plasma membrane

Molecular Function: 1-phosphatidylinositol-3-kinase activity; beta-glucosidase activity; fibroblast growth factor binding; fibroblast growth factor receptor binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: aging; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; phosphoinositide-mediated signaling; positive regulation of bone mineralization; regulation of phosphoinositide 3-kinase cascade

Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial