Typical Testing Data/Standard Curve (for reference only)
Rat Flt-3 ligand ELISA Kit | FLT3LG elisa kit
Rat Flt-3 ligand PicoKine ELISA Kit
Reactivity
Rat
Synonyms
Flt-3 ligand; Rat Flt-3 ligand PicoKine ELISA Kit; Rat Flt-3 ligand; Fms-related tyrosine kinase 3 ligand; Flt3lg; FLT3LG elisa kit
Reactivity
Rat
Specificity
Natural and recombinant Rat Flt3lg
Samples
Cell culture supernates and serum
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FLT3LG elisa kit
Principle of the Assay: The Picokine™ Rat Flt3lg Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Flt3lg with a 96-well strip plate that is pre-coated with antibody specific for Flt3lg. The detection antibody is a biotinylated antibody specific for Flt3lg. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Rat Flt3lg with immunogen: Expression system for standard: NSO, Immunogen sequence: G27-R188. The kit is analytically validated with ready to use reagents. To measure Rat Flt3lg, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Flt3lg in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Flt3lg in the sample.!!Background/Introduction: Fms-related tyrosine kinase 3 ligand (FLT3LG) is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 1q22. Flt3 ligand (FL) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. For example, it is a major growth factor stimulating the growth of dendritic cells.
NCBI and Uniprot Product Information
NCBI GeneID
NCBI Official Synonym Full Names
fms-related tyrosine kinase 3 ligand
NCBI Official Symbol
Flt3lg
NCBI Protein Information
fms-related tyrosine kinase 3 ligand
Protein Family
Similar Products
Product Notes
The Rat FLT3LG (Catalog #AAA1752706) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1752706 ELISA Kit recognizes Rat FLT3LG. It is sometimes possible for the material contained within the vial of "Flt-3 ligand, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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