Rat EphA4 ELISA Kit | EphA4 elisa kit

Rat EphA4 PicoKine ELISA Kit

Cat. Num. AAA1752575

• Gene Names: Epha4; RGD1560587
• Reactivity: Rat
• Synonyms: EphA4; Rat EphA4 PicoKine ELISA Kit; Rat EphA4; Eph receptor A4; Epha4; EphA4 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Epha4
• Sequence Length: 986

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EphA4 elisa kit

Principle of the Assay: The Picokine™ Rat Epha4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Epha4 with a 96-well strip plate that is pre-coated with antibody specific for Epha4. The detection antibody is a biotinylated antibody specific for Epha4. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Rat Epha4 with immunogen: Expression system for standard: NSO, Immunogen sequence: V20-T547. The kit is analytically validated with ready to use reagents. To measure Rat Epha4, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Epha4 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Epha4 in the sample.!!Background/Introduction: EPH receptor A4 (ephrin type-A receptor 4) is a protein that in humans is encoded by the EPHA4 gene. It is mapped to 9q33. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

NCBI and Uniprot Product Information

• NCBI GI #: 241666404
• NCBI GeneID: 316539
• NCBI Accession #: NP_001155883.1
• NCBI GenBank Nucleotide #: NP_001155883.1
• UniProt Accession #: D3ZZK3
• NCBI Official Full Name: ephrin type-A receptor 4
• NCBI Official Synonym Full Names: Eph receptor A4
• NCBI Official Symbol: Epha4
• NCBI Official Synonym Symbols: RGD1560587
• NCBI Protein Information: ephrin type-A receptor 4
• UniProt Protein Name: Protein Epha4
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: Epha4

Uniprot Description

EphA4: a tyrosine kinase receptor of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in hindbrain pattern formation. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels.

Protein type: EC 2.7.10.1; Eph family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: axon; cell projection; cell surface; cytoplasm; dendrite; dendritic spine; early endosome membrane; endoplasmic reticulum; filopodium; Golgi apparatus; integral to plasma membrane; mitochondrial outer membrane; nerve terminal; neuromuscular junction; perikaryon; plasma membrane; postsynaptic density

Molecular Function: ephrin receptor binding; GPI-linked ephrin receptor activity; PH domain binding; protein binding; protein kinase activity; transmembrane-ephrin receptor activity

Biological Process: adult walking behavior; axon guidance; corticospinal tract morphogenesis; glial cell migration; motor axon guidance; negative regulation of axon regeneration; peptidyl-tyrosine phosphorylation; positive regulation of dendrite morphogenesis; positive regulation of JNK activity; protein amino acid autophosphorylation; regulation of astrocyte differentiation; regulation of axonogenesis; regulation of GTPase activity