Flt-3 Ligand Recombinant Protein | Flt-3 recombinant protein

Recombinant Mouse Flt-3 Ligand protein, C-Fc Tag

Cat. Num. AAA1560786

• Gene Names: Flt3; Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Flt-3 Ligand; Recombinant Mouse Flt-3 Ligand protein; C-Fc Tag; Flk-2; Flt-3; Flt-3 recombinant protein

• Host: Mammalian cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Tag: C-Fc Tag
• Species: Mus musculus (Mouse)
• Endotoxin Level: <1.0EU per ug of the protein as determined by the LAL method.
• Protein Construction: A DNA sequence encoding the mouse Flt3 (Gly27-Arg188) was fused with the C-terminal Fc Tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Product Categories/Family for Flt-3 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 122937353
• NCBI GeneID: 14255
• NCBI Accession #: NP_034359.2
• NCBI GenBank Nucleotide #: NM_010229.2
• UniProt Accession #: Q00342
• Molecular Weight: 112,640 Da
• NCBI Official Full Name: receptor-type tyrosine-protein kinase FLT3
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 3
• NCBI Official Symbol: Flt3
• NCBI Official Synonym Symbols: Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04
• NCBI Protein Information: receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; fetal liver kinase 2; tyrosine-protein kinase receptor flk-2
• UniProt Protein Name: Receptor-type tyrosine-protein kinase FLT3
• UniProt Gene Name: Flt3
• UniProt Synonym Gene Names: Flk-2; Flt-3; FLK-2; FLT-3
• UniProt Entry Name: FLT3_MOUSE

Uniprot Description

Function: Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.5 PublicationsManual assertion based on experiment in:Ref.5

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.1 PublicationManual assertion based on experiment in:Ref.10

Enzyme regulation: Present in an inactive conformation in the absence of bound ligand. FLT3LG binding leads to dimerization and activation by autophosphorylation.1 PublicationManual assertion based on experiment in:Ref.10