Testing Data
(Fig: Western blot analysis on mouse brain using anti-Flt3 polyclonal antibody)
anti-Mouse Flt3 Polyclonal Antibody | anti-Flt3 antibody
Flt3 Antibody
Gene Names
Flt3; Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04
Reactivity
Mouse
Applications
Western Blot
Purity
Affinity purified
Synonyms
Flt3; Polyclonal Antibody; Flt3 Antibody; anti-Flt3 antibody
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of N-terminal residues of mouse Flt3.
Purity/Purification
Affinity purified
Form/Format
1*TBS (pH7.4), 0.5%BSA, 25% Glycerol.
Sequence Length
1000
Applicable Applications for anti-Flt3 antibody
Western Blot (WB)
Application Notes
WB: 1:1,000
Preservative
0.05% Sodium Azide.
Cellular Localization
Cell Membrane
Positive Control
Mouse Brain
Preparation and Storage
Store at +4 degree C after thawing. Aliquot store at-20 degree C or-80 degree C. Avoid repeated freeze/thaw cycles.
Testing Data
(Fig: Western blot analysis on mouse brain using anti-Flt3 polyclonal antibody)
Testing Data
(Fig: Western blot analysis on mouse brain using anti-Flt3 polyclonal antibody)
Related Product Information for anti-Flt3 antibody
CD135 is a cytokine receptor expressed on the surface of hematopoietic progenitor cells. FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets. CD135 is a proto-oncogene. Mutations of the Flt3 receptor can lead to the development of leukemia, a cancer of bone marrow hematopoietic progenitors.
References
1. Matthews W., Jordan C.T., Wiegand G.W., Pardoll D., Lemischka I.R.; "A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations."; Cell 65:1.43.1.52.1.91. 2. Maroc N., Rottapel R., Rosnet O., Marchetto S., Lavezzi C., Mannoni P., Birnbaum D., Dubreuil P.; "Biochemical characterization and analysis of the transforming potential of the FLT3.FLK2.receptor tyrosine kinase."; Oncogene 8:909-91.(1.93).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~112kDa
NCBI Official Full Name
receptor-type tyrosine-protein kinase FLT3
NCBI Official Synonym Full Names
FMS-like tyrosine kinase 3
NCBI Official Symbol
Flt3
NCBI Official Synonym Symbols
Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04
NCBI Protein Information
receptor-type tyrosine-protein kinase FLT3
UniProt Protein Name
Receptor-type tyrosine-protein kinase FLT3
Protein Family
UniProt Gene Name
Flt3
UniProt Synonym Gene Names
Flk-2; Flt-3; FLK-2; FLT-3
Uniprot Description
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.
Research Articles on Flt3
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Product Notes
The Flt3 flt3 (Catalog #AAA856907) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Flt3 Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Flt3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1,000. Researchers should empirically determine the suitability of the Flt3 flt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Flt3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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