Mouse FLT3 (Receptor-type tyrosine-protein kinase FLT3) ELISA Kit | FLT3 elisa kit

Mouse FLT3 (Receptor-type tyrosine-protein kinase FLT3) ELISA Kit

Cat. Num. AAA7606719

• Gene Names: Flt3; Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04
• Reactivity: Mouse
• Synonyms: FLT3 (Receptor-type tyrosine-protein kinase FLT3); Mouse FLT3 (Receptor-type tyrosine-protein kinase FLT3) ELISA Kit; FLT3 elisa kit

• Reactivity: Mouse
• Sequence Length: 1000

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLT3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- FLT3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-FLT3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FLT3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FLT3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 803341901
• NCBI GeneID: 14255
• UniProt Accession #: Q00342
• Molecular Weight: 113,496 Da
• NCBI Official Full Name: Receptor-type tyrosine-protein kinase FLT3
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 3
• NCBI Official Symbol: Flt3
• NCBI Official Synonym Symbols: Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04
• NCBI Protein Information: receptor-type tyrosine-protein kinase FLT3
• UniProt Protein Name: Receptor-type tyrosine-protein kinase FLT3
• Protein Family: Receptor-type tyrosine-protein kinase
• UniProt Gene Name: Flt3
• UniProt Synonym Gene Names: Flk-2; Flt-3; FLK-2; FLT-3

Uniprot Description

FLT3: a receptor tyrosine kinase of the PDGFR family that binds the FL cytokine. FLT3 -/- mice have an impaired developmental capacity of primitive hematopoietic progenitor cells of all lineages with the greatest impact on lymphopoietic precursors. Activating mutations found in one third of cases of acute myeloid leukemia (AML), as well as in acute lymphoblastic leukemia, acute promyelocytic leukemia and myelodysplastic syndrome. Inhibitors: Sutent and PKC412.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; PDGFR family; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 5 86.88 cM|5 G3

Cellular Component: cell surface; cytoplasm; cytosol; endoplasmic reticulum; external side of plasma membrane; nucleus; plasma membrane; protein complex

Molecular Function: glucocorticoid receptor binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; phosphoinositide 3-kinase binding; protein binding; protein complex binding; protein self-association; protein-tyrosine kinase activity; ubiquitin protein ligase binding

Biological Process: antigen processing and presentation; B cell differentiation; cytokine and chemokine mediated signaling pathway; hemopoiesis; homeostasis of number of cells within a tissue; leukocyte homeostasis; lymph node development; lymphocyte differentiation; lymphocyte proliferation; lymphoid progenitor cell differentiation; myeloid progenitor cell differentiation; negative regulation of B cell differentiation; negative regulation of cell proliferation; negative regulation of interleukin-6 production; negative regulation of tumor necrosis factor production; positive regulation of interferon-alpha production; positive regulation of interferon-gamma production; positive regulation of interleukin-12 production; positive regulation of multicellular organism growth; positive regulation of protein amino acid phosphorylation; post-embryonic development; pro-B cell differentiation; pro-T cell differentiation; protein amino acid autophosphorylation; spleen development