FLT-3 Recombinant Protein | Flt3 recombinant protein

Human CellExp FLT-3, Mouse Recombinant

Cat. Num. AAA8400102

• Gene Names: Flt3; Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04
• Purity: >90%
• Synonyms: FLT-3; Human CellExp FLT-3; Mouse Recombinant; Flt-3; Flk-2; STK-1; CD135; FLK2; Flt3 recombinant protein

• Host: HEK293 cells
• Purity/Purification: >90%
• Form/Format: Lyophilized powder. Lyophilized from 0.22um filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Sequence: Asn28-Ser544

• Gene Source: Mouse
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Reconstitution Instruction: Reconstitute in sterile deionized water to the desired protein concentration.
• Tag Note: This protein carries a polyhistidine tag at the C-terminus.
• Preparation and Storage: Store at -20 degree C; Centrifuge the vial prior to opening.; Shelf life: ~12 months

SDS-Page

Related Product Information for Flt3 recombinant protein

Flt-3 (Receptor-type tyrosine-protein kinase FLT3) is also known as FLK-2 (Fetal liver kinase-2), STK-1 (Stem cell tyrosine kinase 1), CD135. FLT3 is a cytokine receptor which belongs to the receptor tyrosine kinase class III. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

Product Categories/Family for Flt3 recombinant protein

Proteins; Enzymes; Proteins & Enzymes (A-Z); Stem Cell Research Tools; Cytokines & Growth Factors; For Hematopoietic Stem Cells; Cytokines; Cytokines & Growth Factors (A-Z); Human cell expressed recombinant proteins

NCBI and Uniprot Product Information

• NCBI GI #: 122937353
• NCBI GeneID: 14255
• NCBI Accession #: NP_034359.2
• NCBI GenBank Nucleotide #: NM_010229.2
• UniProt Accession #: Q00342
• Molecular Weight: 112,640 Da
• NCBI Official Full Name: receptor-type tyrosine-protein kinase FLT3
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 3
• NCBI Official Symbol: Flt3
• NCBI Official Synonym Symbols: Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04
• NCBI Protein Information: receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; fetal liver kinase 2; tyrosine-protein kinase receptor flk-2
• UniProt Protein Name: Receptor-type tyrosine-protein kinase FLT3
• Protein Family: Receptor-type tyrosine-protein kinase
• UniProt Gene Name: Flt3
• UniProt Synonym Gene Names: Flk-2; Flt-3; FLK-2; FLT-3
• UniProt Entry Name: FLT3_MOUSE

Uniprot Description

Function: Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.5 PublicationsManual assertion based on experiment in:Ref.5

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.1 PublicationManual assertion based on experiment in:Ref.10

Enzyme regulation: Present in an inactive conformation in the absence of bound ligand. FLT3LG binding leads to dimerization and activation by autophosphorylation.1 PublicationManual assertion based on experiment in:Ref.10