Rabbit anti-Mouse Flt3 Polyclonal Antibody | anti-Flt3 antibody

Flt3, NT (Flt3, Flk-2, Flt-3, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase 2, Fms-like tyrosine kinase 3, Tyrosine-protein kinase receptor flk-2, CD135) (Azide free) (HRP)

Cat. Num. AAA6299449

• Gene Names: Flt3; Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Flt3; Polyclonal Antibody; NT (Flt3; Flk-2; Flt-3; Receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; Fetal liver kinase 2; Fms-like tyrosine kinase 3; Tyrosine-protein kinase receptor flk-2; CD135) (Azide free) (HRP); CD135; anti-Flt3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Flt3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Flt3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-181 amino acids from the N-terminal region of Mouse Flt3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Flt3 Antibody (N-term) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the Flt3 antibody detected the Flt3 protein (arrow).)

Related Product Information for anti-Flt3 antibody

Flt3 is a receptor for the FL cytokine. Has a tyrosine-protein kinase activity.

Product Categories/Family for anti-Flt3 antibody

Antibodies; Protein Kinases

References

Rathinam, C., et al. Cancer Cell 18(4):341-352(2010). DeBoy, C.A., et al. Exp. Mol. Pathol. 89(2):109-116(2010). Mkonyi, L.E., et al. Am. J. Physiol. Heart Circ. Physiol. 299 (2), H275-H283 (2010): Carotta, S., et al. Immunity 32(5):628-641(2010). Eidenschenk, C., et al. Proc. Natl. Acad. Sci. U.S.A. 107(21):9759-9764(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 122937353
• NCBI GeneID: 14255
• NCBI Accession #: NP_034359.2
• NCBI GenBank Nucleotide #: NM_010229.2
• UniProt Accession #: Q00342
• Molecular Weight: 112,640 Da
• NCBI Official Full Name: receptor-type tyrosine-protein kinase FLT3
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 3
• NCBI Official Symbol: Flt3
• NCBI Official Synonym Symbols: Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04
• NCBI Protein Information: receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; fetal liver kinase 2; tyrosine-protein kinase receptor flk-2
• UniProt Protein Name: Receptor-type tyrosine-protein kinase FLT3
• Protein Family: Flt3 receptor-interacting lectin
• UniProt Gene Name: Flt3
• UniProt Synonym Gene Names: Flk-2; Flt-3; FLK-2; FLT-3
• UniProt Entry Name: FLT3_MOUSE

Uniprot Description

Function: Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.5 PublicationsManual assertion based on experiment in:Ref.5

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.1 PublicationManual assertion based on experiment in:Ref.10

Enzyme regulation: Present in an inactive conformation in the absence of bound ligand. FLT3LG binding leads to dimerization and activation by autophosphorylation.1 PublicationManual assertion based on experiment in:Ref.10