Human peroxiredoxin 1 ELISA Kit | PRDX1 elisa kit

Human Peroxiredoxin-1, PRDX1 ELISA Kit

Cat. Num. AAA928497

• Gene Names: PRDX1; PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A
• Reactivity: Human
• Synonyms: peroxiredoxin 1; Human Peroxiredoxin-1; PRDX1 ELISA Kit; Human Peroxiredoxin-1 (PRDX1) ELISA kit; MSP23; NKEFA; PAG; PAGA; PAGB; PRX1; PRXI; TDPX2; natural killer-enhancing factor A; proliferation-associated gene A; thioredoxin-dependent peroxide reductase 2; PRDX1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human PRDX1. No significant cross-reactivity or interference between human PRDX1 and analogues was observed.
• Sequence Length: 199

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 3.12 ng/ml -200 ng/ml.
• Sensitivity: The minimum detectable dose of human PRDX1 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRDX1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRDX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRDX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 320461711
• NCBI GeneID: 5052
• NCBI Accession #: NP_001189360.1
• NCBI GenBank Nucleotide #: NM_001202431.1
• UniProt Accession #: Q06830; P35703; Q2V576; Q5T154; Q5T155; B5BU26; D3DPZ8
• Molecular Weight: 22,110 Da
• NCBI Official Full Name: peroxiredoxin-1
• NCBI Official Synonym Full Names: peroxiredoxin 1
• NCBI Official Symbol: PRDX1
• NCBI Official Synonym Symbols: PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A
• NCBI Protein Information: peroxiredoxin-1; thioredoxin peroxidase 2; proliferation-associated gene A; natural killer-enhancing factor A; proliferation-associated gene protein; natural killer cell-enhancing factor A; thioredoxin-dependent peroxide reductase 2
• UniProt Protein Name: Peroxiredoxin-1
• Protein Family: Peroxiredoxin
• UniProt Gene Name: PRDX1
• UniProt Synonym Gene Names: PAGA; PAGB; TDPX2; NKEF-A; PAG
• UniProt Entry Name: PRDX1_HUMAN

NCBI Description

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

PRDX1: a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). May have a proliferative effect and play a role in cancer development or progression.

Protein type: Nuclear receptor co-regulator; Oxidoreductase; EC 1.11.1.15

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: peroxisomal matrix; extracellular space; mitochondrial matrix; cytoplasm; nucleolus; melanosome; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; peroxidase activity; thioredoxin peroxidase activity; heme binding

Biological Process: erythrocyte homeostasis; regulation of stress-activated MAPK cascade; retinal homeostasis; cell proliferation; response to reactive oxygen species; removal of superoxide radicals; natural killer cell mediated cytotoxicity; hydrogen peroxide catabolic process; skeletal development; regulation of NF-kappaB import into nucleus