Rat surfactant protein B ELISA Kit | SFTPB elisa kit

Rat Pulmonary Surfactant-associated protein B, SP-B ELISA Kit

Cat. Num. AAA703167

• Gene Names: SFTPB; SP-B; SFTP3
• Reactivity: Rat
• Synonyms: surfactant protein B; Rat Pulmonary Surfactant-associated protein B; SP-B ELISA Kit; Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit; PSP-B; SFTB3; SFTP3; SMDP1; SP-B; OTTHUMP00000203253; Pulmonary surfactant-associated protein B; 18kD; surfactant; pulmonary-associated protein B; SFTPB elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat SP-B. No significant cross-reactivity or interference between rat SP-B and analogues was observed.
• Sequence Length: 369

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 3.12 pg/ml-200 pg/ml.
• Sensitivity: The minimum detectable dose of rat SP-B is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SFTPB elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 126723505
• NCBI GeneID: 100009194
• NCBI Accession #: NP_001075812.1
• NCBI GenBank Nucleotide #: NM_001082343.1
• UniProt Accession #: P15285; P79333
• Molecular Weight: 40,610 Da
• NCBI Official Full Name: pulmonary surfactant-associated protein B
• NCBI Official Symbol: SFTPB
• NCBI Official Synonym Symbols: SP-B; SFTP3
• NCBI Protein Information: pulmonary surfactant-associated protein B; 6 kDa protein; pulmonary surfactant-associated proteolipid SPL(Phe)
• UniProt Protein Name: Pulmonary surfactant-associated protein B
• Protein Family: Pulmonary surfactant-associated protein
• UniProt Gene Name: SFTPB
• UniProt Synonym Gene Names: SFTP3; SP-B
• UniProt Entry Name: PSPB_RABIT

Uniprot Description

Function: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.