Rabbit anti-Human ATP6V1F Polyclonal Antibody | anti-ATP6V1F antibody

ATP6V1F, CT (ATP6V1F, ATP6S14, VATF, V-type proton ATPase subunit F, V-ATPase 14kD subunit, Vacuolar proton pump subunit F) (APC)

Cat. Num. AAA6276701

• Gene Names: ATP6V1F; VATF; Vma7; ATP6S14
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATP6V1F; Polyclonal Antibody; CT (ATP6V1F; ATP6S14; VATF; V-type proton ATPase subunit F; V-ATPase 14kD subunit; Vacuolar proton pump subunit F) (APC); Vacuolar proton pump subunit F; anti-ATP6V1F antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 119

• Applicable Applications for anti-ATP6V1F antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ATP6V1F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-111 amino acids from the C-terminal region of human ATP6V1F.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ATP6V1F Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ATP6V1F antibody detected the ATP6V1F protein (arrow).)

Product Categories/Family for anti-ATP6V1F antibody

Antibodies; Enzymes; ATPase

References

Supino, R., et al. Ann. N. Y. Acad. Sci. 1171, 606-616 (2009): Smith, A.N., et al. J. Bioenerg. Biomembr. 40(4):371-380(2008). Morel, N. Biol. Cell 95(7):453-457(2003). Smith, A.N., et al. Mol. Cell 12(4):801-803(2003). Kawasaki-Nishi, S., et al. FEBS Lett. 545(1):76-85(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 20357547
• NCBI GeneID: 9296
• NCBI Accession #: NP_004222.2
• NCBI GenBank Nucleotide #: NP_004222.2
• UniProt Accession #: Q16864
• NCBI Official Full Name: V-type proton ATPase subunit F isoform 1
• NCBI Official Synonym Full Names: ATPase H+ transporting V1 subunit F
• NCBI Official Symbol: ATP6V1F
• NCBI Official Synonym Symbols: VATF; Vma7; ATP6S14
• NCBI Protein Information: V-type proton ATPase subunit F
• UniProt Protein Name: V-type proton ATPase subunit F
• UniProt Gene Name: ATP6V1F
• UniProt Synonym Gene Names: ATP6S14; VATF; V-ATPase subunit F
• UniProt Entry Name: VATF_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V1F: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase F subunit family.

Protein type: Hydrolase

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: cytosol; membrane; vacuolar proton-transporting V-type ATPase complex

Molecular Function: ATPase activity, coupled to transmembrane movement of ions; protein binding

Biological Process: insulin receptor signaling pathway; transferrin transport