Fibrillin-1/Asprosin Recombinant Protein | Fbn1 recombinant protein

Recombinant Mouse Fibrillin-1/Asprosin Protein (N-8His)

Cat. Num. AAA2553495

• Gene Names: Fbn1; Tsk; Fib-1; AI536462; B430209H23
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: Fibrillin-1/Asprosin; Recombinant Mouse Fibrillin-1/Asprosin Protein (N-8His); Fibrillin-1; Fbn1; Asprosin; Fbn-1; Fbn1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution of PBS, pH7.4.
• Sequence Positions: Ser2732 - His2871
• Sequence Length: 2873

• Species: Mouse
• Accession: AAA56840.1
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Tag: N-8His
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex.; It is recommended that sterile water(66ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C . Reconstituted protein solution can be stored at 4-8°C for up to 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for up to 3 months.; Shipping: This product is provided as lyophilized powder which is shipped with blue ice.

SDS-PAGE

Related Product Information for Fbn1 recombinant protein

Asprosin is a protein hormone that is produced by white adipose tissue in mammals (and potentially by other tissues), which is then transported to the liver and stimulates it to release glucose into the blood stream. In the liver asprosin activates rapid glucose release by a cAMP-dependent pathway. The glucose release by the liver into the blood stream is vital for brain function and survival during fasting. People with neonatal progeroid syndrome lack asprosin, while people with insulin resistance have it in abundance. In animal tests asprosin showed potential for treating type 2 diabetes. When antibodies targeting asprosin were injected into diabetic mice, blood glucose and insulin levels improved.

NCBI and Uniprot Product Information

• NCBI GI #: 118197277
• NCBI GeneID: 14118
• NCBI Accession #: NP_032019.2
• NCBI GenBank Nucleotide #: NM_007993.2
• UniProt Accession #: Q61554; Q60826; A2AQ53
• Molecular Weight: Molecular Mass: 16.9 kDa; Actual Protein Molecular Mass: 30 kDa
• NCBI Official Full Name: fibrillin-1 preproprotein
• NCBI Official Synonym Full Names: fibrillin 1
• NCBI Official Symbol: Fbn1
• NCBI Official Synonym Symbols: Tsk; Fib-1; AI536462; B430209H23
• NCBI Protein Information: fibrillin-1
• UniProt Protein Name: Fibrillin-1
• Protein Family: Fibrillin
• UniProt Gene Name: Fbn1
• UniProt Synonym Gene Names: Fbn-1

NCBI Description

This gene encodes a member of the fibrillin family of proteins. The encoded preproprotein is proteolytically processed to generate two proteins including the extracellular matrix component fibrillin-1 and the protein hormone asprosin. Fibrillin-1 is an extracellular matrix glycoprotein that serves as a structural component of calcium-binding microfibrils. Asprosin, secreted by white adipose tissue, has been shown to regulate glucose homeostasis. Homozygous knockout mice for this gene exhibit impaired aortic development and early postnatal death, which was attributed to a deficiency in the fibrillin-1 protein. Mice with a hypomorphic allele of this gene exhibit impaired glucose homeostasis, likely due to a reduction in serum asprosin levels. [provided by RefSeq, Apr 2016]

Uniprot Description

Fibrillin-1: Structural component of the 10-12 nm diameter microfibrils of the extracellular matrix, which conveys both structural and regulatory properties to load-bearing connective tissues. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. In tissues such as the lung, blood vessels and skin, microfibrils form the periphery of the elastic fiber, acting as a scaffold for the deposition of elastin. In addition, microfibrils can occur as elastin-independent networks in tissues such as the ciliary zonule, tendon, cornea and glomerulus where they provide tensile strength and have anchoring roles. Fibrillin-1 also plays a key role in tissue homeostasis through specific interactions with growth factors, such as the bone morphogenetic proteins (BMPs), growth and differentiation factors (GDFs) and latent transforming growth factor-beta-binding proteins (LTBPs), cell-surface integrins and other extracellular matrix protein and proteoglycan components (). Regulates osteoblast maturation by controlling TGF-beta bioavailability and calibrating TGF-beta and BMP levels, respectively (PubMed:20855508). Negatively regulates osteoclastogenesis by binding and sequestering an osteoclast differentiation and activation factor TNFSF11. This leads to disruption of TNFSF11-induced Ca2+ signaling and impairment of TNFSF11-mediated nuclear translocation and activation of transcription factor NFATC1 which regulates genes important for osteoclast differentiation and function (PubMed:24039232). Mediates cell adhesion via its binding to cell surface receptors integrins ITGAV:ITGB3 and ITGA5:ITGB1. Binds heparin and this interaction plays an important role in the assembly of microfibrils ().