Interferon Gamma (IFNg) Recombinant Protein | IFNg recombinant protein

Recombinant Interferon Gamma (IFNg)

Cat. Num. AAA2011516

• Gene Names: Ifng; Ifg; IFN-g
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Interferon Gamma (IFNg); Recombinant Interferon Gamma (IFNg); IFNg recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with a N-terminal His-tag, its sequence is listed below.; MGHHHHHHSGSEF-HGTVIESL ESLNNYFNSS GIDVEEKSLF LDIWRNWQKD GDMKILQSQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT TFFSNSKAKK DAFMSIAKFE VNNPQVQRQA FNELIRVVHQ LLPESSLRKR KRSRC
• Sequence Length: 155

• Applicable Applications for IFNg recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: His23~Cys155 (Accession # P01580) with a N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 33468859
• NCBI GeneID: 15978
• NCBI Accession #: NP_032363.1
• NCBI GenBank Nucleotide #: NM_008337.3
• UniProt Accession #: P01580; Q542B8
• Molecular Weight: 17.0kDa
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon gamma
• NCBI Official Symbol: Ifng
• NCBI Official Synonym Symbols: Ifg; IFN-g
• NCBI Protein Information: interferon gamma; IFN-gamma; gamma interferon
• UniProt Protein Name: Interferon gamma
• Protein Family: Interferon
• UniProt Gene Name: Ifng
• UniProt Synonym Gene Names: IFN-gamma
• UniProt Entry Name: IFNG_MOUSE

NCBI Description

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]

Uniprot Description

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Secreted; Membrane protein, integral; Cytokine; Secreted, signal peptide

Cellular Component: extracellular space; cell; extracellular region; intracellular; external side of plasma membrane

Molecular Function: protein binding; interferon-gamma receptor binding; cytokine activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of cell adhesion; positive regulation of nitric oxide biosynthetic process; positive regulation of interleukin-23 production; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; negative regulation of epithelial cell differentiation; positive regulation of interleukin-1 beta secretion; positive regulation of killing of cells of another organism; positive regulation of membrane protein ectodomain proteolysis; regulation of growth; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; neutrophil chemotaxis; positive regulation of synaptic transmission, cholinergic; CD8-positive, alpha-beta T cell differentiation during immune response; negative regulation of myelination; response to virus; defense response to protozoan; positive regulation of tumor necrosis factor production; inflammatory cell apoptosis; positive regulation of tyrosine phosphorylation of Stat1 protein; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-12 production; negative regulation of smooth muscle cell proliferation; apoptosis; positive regulation of interleukin-6 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; sensory perception of mechanical stimulus; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; regulation of immune response; adaptive immune response; unfolded protein response; humoral immune response; antigen processing and presentation; positive regulation of chemokine biosynthetic process; negative regulation of interleukin-17 production; positive regulation of interleukin-12 biosynthetic process; protein import into nucleus, translocation; immune response; positive regulation of neuron differentiation; regulation of insulin secretion