TFRC recombinant protein

Recombinant Human TFRC Protein (N-His)

Cat. Num. AAA2557105

• Gene Names: TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: TFRC; Recombinant Human TFRC Protein (N-His); Transferrin receptor protein 1; TR; TfR; Trfr; T9; p90; TFRC recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, pH7.4.
• Sequence: Leu101-Phe760
• Sequence Length: 760

• Species: Human
• Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Tag: N-6His
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex.; It is recommended that sterile water(50ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

SDS-PAGE

Related Product Information for TFRC recombinant protein

Transferrin receptor protein 1 (TFRC) belongs to the peptidase M28 family that is synthesized as a 172 amino acid (aa). TFRC regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. It binds one transferrin or HFE molecule per subunit and binds the HLA class II histocompatibility antigen, DR1. It Interacts with SH3BP3 and STEAP3, facilitates TFRC endocytosis in erythroid precursor cells. Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. It positively regulates T and B cell proliferation through iron uptake.

NCBI and Uniprot Product Information

• NCBI GI #: 189458819
• NCBI GeneID: 7037
• NCBI Accession #: NP_001121620.1
• NCBI GenBank Nucleotide #: NP_001121620.1
• UniProt Accession #: P02786
• Molecular Weight: Molecular Mass: 74.9kDa; Actual Protein Molecular Mass: 80-90kDa
• NCBI Official Full Name: transferrin receptor protein 1 isoform 1
• NCBI Official Synonym Full Names: transferrin receptor
• NCBI Official Symbol: TFRC
• NCBI Official Synonym Symbols: T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46
• NCBI Protein Information: transferrin receptor protein 1
• UniProt Protein Name: Transferrin receptor protein 1
• Protein Family: Transferrin receptor protein
• UniProt Gene Name: TFRC
• UniProt Synonym Gene Names: TR; TfR; TfR1; Trfr; sTfR
• UniProt Entry Name: TFR1_HUMAN

NCBI Description

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Uniprot Description

TfR: the transferrin receptor. Regulates the cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied receptors into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system.

Protein type: Membrane protein, integral; Receptor, misc.; Cell surface

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasmic membrane-bound vesicle; extracellular region; coated pit; recycling endosome; membrane; perinuclear region of cytoplasm; melanosome; plasma membrane; vesicle; endosome; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; transferrin receptor activity; double-stranded RNA binding

Biological Process: receptor-mediated endocytosis; viral reproduction; cellular iron ion homeostasis; positive regulation of bone resorption; transferrin transport; osteoclast differentiation; regulation of cell growth; transmembrane transport; regulation of cell proliferation