Eukaryotes Protein Synthesis Assay Kit

EZClick Global Protein Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence

Cat. Num. AAA849744

• Reactivity: Eukaryotes
• Synonyms: Protein Synthesis; EZClick Global Protein Synthesis Assay Kit (FACS/Microscopy); Red Fluorescence; Protein Synthesis assay kit

• Reactivity: Eukaryotes

• Samples: Suspension or adherent cell cultures
• Detection Method: Flow Cytometry: (Ex/Em (440/490nm and 530/590nm) and Fluorescence Microscopy
• Features & Benefits: Simple, fast, does not require lengthy incubation times; This assay provides a convenient and accurate procedure to measure de novo protein synthesized in biological samples
• Preparation and Storage: Store at -20 degree C; Shelf life: ~12 months

Immunocytochemistry/Immunofluorescence (ICC/IF))

Immunocytochemistry/Immunofluorescence (ICC/IF))

Related Product Information for Protein Synthesis assay kit

Cells generate a complete set of proteins during division. Protein synthesis is a tightly regulated process and many critical controls in gene expression occur at the level of translation to ensure that production of specific cellular proteins is quickly turned on/off under specific conditions (heat shock, starvation, etc.). Protein synthesis is essential in cell growth, proliferation, signaling, differentiation or death; therefore, the identity and amount of the synthesized proteins are critical parameters in determining the physiological state of the cell. Methods enabling detection and characterization of nascent proteins, or changes in spatial and temporal protein expression/degradation patterns during disease, drug treatments or environmental changes are important tools in assessment of cytotoxicity. EZClick Protein Synthesis Monitoring Assay Kit utilizes a novel and robust chemical method based on an alkyne containing and cell-permeable analog of puromycin, O-Propargyl-puromycin (OP-puro). Once inside the cell, OP-puro stops translation by forming covalent conjugates with nascent polypeptide chains. Truncated polypeptides are rapidly turned over by the proteasome and can be detected based on a click reaction with the fluorescent azide. Unlike methionine analogs, OP-puro does not require methionine-free conditions and can be used to label nascent proteins directly in the cell culture. Our kit provides sufficient materials for 100 assays to detect nascent proteins synthesized under various physiological conditions, and Cycloheximide, an inhibitor of protein synthesis that serves as an experimental control

Product Categories/Family for Protein Synthesis assay kit

Cell Proliferation; Viability & Cytotoxicity; Cell Proliferation Assay Kits