Western Blot (WB)
Rabbit anti-Mouse, Rat NEDD4 Polyclonal Antibody | anti-NEDD4 antibody
NEDD4 Antibody Affinity Purified
ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:250
Western Blot (WB)
Western Blot (WB)
E3 ubiquitin-protein ligase;Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding
NCBI and Uniprot Product Information
NCBI Description
may regulate function of the amiloride-sensitive epithelial sodium channel ENaC [RGD, Feb 2006]
Uniprot Description
NEDD4: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR- 2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Interacts with UBE2D2. Binds SCNN1A, SCNN1B and SCNN1G. Binds, in vitro, through the WW2 and WW3 domains, to neural isoforms of ENAH that contain the PPSY motif. Interacts with BEAN1, LITAF, RNF11, WBP1, WBP2, TMEPAI and PRRG2. Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase and may induce its recruitment to exosomes. Interaction with PTEN is questionable according to PubMed:18562292. Interacts with viral proteins that contain a late- budding motif P-P-P-Y. This interaction is essential for viral particle budding of a lot of retroviruses, like HTLV-1 Gag and MLV Gag. Interacts (via C2 domain) with GRB10 (via SH2 domain). Interacts with ERBB4. Interacts with TNIK; the interaction is direct, allows the TNIK-dependent recruitment of RAP2A and its ubiquitination by NEDD4. Interacts (via WW3 domain) with TNK2; EGF promotes this interaction. Interacts (via WW3 domain) with FGFR1 (via C-terminus). Activated by NDFIP1- and NDFIP2-binding. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.-; Nuclear receptor co-regulator; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase
Cellular Component: cell cortex; chromatin; cytoplasm; cytosol; Golgi apparatus; lipid raft; membrane; microvillus; nucleus; perinuclear region of cytoplasm; plasma membrane; ubiquitin ligase complex
Molecular Function: beta-2 adrenergic receptor binding; ligase activity; phosphoserine binding; phosphothreonine binding; protein binding; protein C-terminus binding; protein domain specific binding; sodium channel inhibitor activity; ubiquitin binding; ubiquitin-protein ligase activity
Biological Process: adaptive immune response; blood vessel morphogenesis; glucocorticoid receptor signaling pathway; immune response; lysosomal transport; negative regulation of transcription from RNA polymerase II promoter; negative regulation of vascular endothelial growth factor receptor signaling pathway; neurite development; neuromuscular junction development; positive regulation of nucleocytoplasmic transport; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein catabolic process; progesterone receptor signaling pathway; protein monoubiquitination; protein targeting to lysosome; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; receptor catabolic process; receptor internalization; regulation of dendrite morphogenesis; regulation of membrane potential; regulation of synapse organization and biogenesis; response to denervation involved in regulation of muscle adaptation; T cell activation; transmission of virus; ubiquitin-dependent protein catabolic process via the multivesicular body pathway
Research Articles on NEDD4
Similar Products
Product Notes
The NEDD4 nedd4 (Catalog #AAA540045) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEDD4 Antibody Affinity Purified reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NEDD4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), IMM, Western Blot (WB). Dot Blot: 1:10,000 ELISA: 1:10,000 Immunoprecipitation: 1:200 Western Blot: 1:250. Researchers should empirically determine the suitability of the NEDD4 nedd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEDD4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
