Tumour Type M2 Pyruvate Kinase Active Protein | mPKM2 active protein
Recombinant Mouse Tumour Type M2 Pyruvate Kinase
Gene Names
Pkm; Pk3; Pk-2; Pk-3; Pkm2; AA414905; AL024370; AL024424
Purity
Greater than 85% as determined by SDS-PAGE
Synonyms
Tumour Type M2 Pyruvate Kinase; Recombinant Mouse Tumour Type M2 Pyruvate Kinase; Pyruvate kinase isozymes M1/M2; EC 2.7.1.40; Pyruvate kinase muscle isozyme; Pyruvate kinase 2/3; Cytosolic thyroid hormone-binding protein; CTHBP; THBP1; M2PK; PKM2; PK3; PK2; PKM; TCB; OIP3; MGC3932; Tumor Type M2 Pyruvate Kinase; PKM2 Mouse; Tumour Type M2 Pyruvate Kinase Mouse Recombinant; mPKM2 active protein
Host
Escherichia Coli
Purity/Purification
Greater than 85% as determined by SDS-PAGE
Form/Format
Sterile filtered clear solution
Protein solution (0.5mg/ml) containing 20mM Tris-HCl (pH 8.5), 0.2M NaCl, 1mM DTT and 30% glycerol
Protein solution (0.5mg/ml) containing 20mM Tris-HCl (pH 8.5), 0.2M NaCl, 1mM DTT and 30% glycerol
Sequence
MGSSHHHHHH SSGLVPRGSH MGS MPKPHSE AGTAFIQTQQ LHAAMADTFL EHMCRLDIDS APITARNTGI ICTIGPASRS VEMLKEMIKS GMNVARLNFS HGTHEYHAET IKNVREATES FASDPILYRP VAVALDTKGP EIRTGLIKGS GTAEVELKKG ATLKITLDNA YMEKCDENIL WLDYKNICKV VEVGSKIYVD DGLISLQVKE KGADFLVTEV ENGGSLGSKK GVNLPGAAVD LPAVSEKDIQ DLKFGVEQDV DMVFASFIRK AADVHEVRKV LGEKGKNIKI ISKIENHEGV RRFDEILEAS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC NRAGKPVICA TQMLESMIKK PRPTRAEGSD VANAVLDGAD CIMLSGETAK GDYPLEAVRM QHLIAREAEA AIYHLQLFEE LRRLAPITSD PTEAAAVGAV EASFKCCSGA IIVLTKSGRS AHQVARYRPR APIIAVTRNP QTARQAHLYR GIFPVLCKDA VLNAWAEDVD LRVNLAMDVG KARGFFKKGD VVIVLTGWRP GSGFTNTMRV VPVP
Sequence Length
531
Biological Activity
Specific activity: > 500 units/mg. One unit will convert 1 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37 degree C
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at-20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles
Related Product Information for mPKM2 active protein
Description: PKM2 Mouse Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 554 amino acids (1-531 a.a) and having a molecular mass of 60.2kDa. PKM2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction: Pyruvate kinase is a key enzyme in the glycolytic pathway. The M2 isoenzyme of pyruvate kinase is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. The marker is useful for measuring disease activity, sensitivity to chemotherapy and recurrence.
Introduction: Pyruvate kinase is a key enzyme in the glycolytic pathway. The M2 isoenzyme of pyruvate kinase is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. The marker is useful for measuring disease activity, sensitivity to chemotherapy and recurrence.
Product Categories/Family for mPKM2 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,985 Da
NCBI Official Full Name
Pyruvate kinase PKM
NCBI Official Synonym Full Names
pyruvate kinase, muscle
NCBI Official Symbol
Pkm
NCBI Official Synonym Symbols
Pk3; Pk-2; Pk-3; Pkm2; AA414905; AL024370; AL024424
NCBI Protein Information
pyruvate kinase PKM
UniProt Protein Name
Pyruvate kinase PKM
UniProt Gene Name
Pkm
UniProt Synonym Gene Names
Pk3; Pkm2; Pykm
Uniprot Description
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation ().
Research Articles on mPKM2
Similar Products
Product Notes
The mPKM2 pkm (Catalog #AAA147152) is an Active Protein produced from Escherichia Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGS MPKPHSE AGTAFIQTQQ LHAAMADTFL EHMCRLDIDS APITARNTGI ICTIGPASRS VEMLKEMIKS GMNVARLNFS HGTHEYHAET IKNVREATES FASDPILYRP VAVALDTKGP EIRTGLIKGS GTAEVELKKG ATLKITLDNA YMEKCDENIL WLDYKNICKV VEVGSKIYVD DGLISLQVKE KGADFLVTEV ENGGSLGSKK GVNLPGAAVD LPAVSEKDIQ DLKFGVEQDV DMVFASFIRK AADVHEVRKV LGEKGKNIKI ISKIENHEGV RRFDEILEAS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC NRAGKPVICA TQMLESMIKK PRPTRAEGSD VANAVLDGAD CIMLSGETAK GDYPLEAVRM QHLIAREAEA AIYHLQLFEE LRRLAPITSD PTEAAAVGAV EASFKCCSGA IIVLTKSGRS AHQVARYRPR APIIAVTRNP QTARQAHLYR GIFPVLCKDA VLNAWAEDVD LRVNLAMDVG KARGFFKKGD VVIVLTGWRP GSGFTNTMRV VPVP. It is sometimes possible for the material contained within the vial of "Tumour Type M2 Pyruvate Kinase, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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