SDS-Page
Pkm active protein
Pkm, 1-531aa, Mouse, His-tag, E Coli (Bioactivity Validated)
Gene Names
Pkm; Pk3; Pk-2; Pk-3; Pkm2; AA414905; AL024370; AL024424
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
Pkm; 1-531aa; Mouse; His-tag; E Coli (Bioactivity Validated); Pyruvate kinase PKM isoform M2; AA414905; AL024370; AL024424; Pk-2; Pk-3; Pk3; Pkm2; Pkm active protein
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl (pH 8.5) containing 0.2M NaCl, 1mM DTT, 30% glycerol
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMPKPHSE AGTAFIQTQQ LHAAMADTFL EHMCRLDIDS APITARNTGI ICTIGPASRS VEMLKEMIKS GMNVARLNFS HGTHEYHAET IKNVREATES FASDPILYRP VAVALDTKGP EIRTGLIKGS GTAEVELKKG ATLKITLDNA YMEKCDENIL WLDYKNICKV VEVGSKIYVD DGLISLQVKE KGADFLVTEV ENGGSLGSKK GVNLPGAAVD LPAVSEKDIQ DLKFGVEQDV DMVFASFIRK AADVHEVRKV LGEKGKNIKI ISKIENHEGV RRFDEILEAS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC NRAGKPVICA TQMLESMIKK PRPTRAEGSD VANAVLDGAD CIMLSGETAK GDYPLEAVRM QHLIAREAEA AIYHLQLFEE LRRLAPITSD PTEAAAVGAV EASFKCCSGA IIVLTKSGRS AHQVARYRPR APIIAVTRNP QTARQAHLYR GIFPVLCKDA VLNAWAEDVD LRVNLAMDVG KARGFFKKGD VVIVLTGWRP GSGFTNTMRV VPVP
Sequence Length
531
Applicable Applications for Pkm active protein
SDS-PAGE
Antigen Species
Mouse
Tag Information
His-tag
Biological Activity
Specific activity: > 50,000 pmol/min/ug. One unit will convert 1.0 pmole of phospho (enol)pyruvate to pyruvate per minute at pH 7.5 at 37C
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for Pkm active protein
Pkm, also known as pyruvate kinase PKM isoform M2, is an isoenzyme of the glycolytic enzyme pyruvate kinase. This protein catalyzes the production of pyruvate and ATP from phosphoenolpyruvate. Pkm interacting with Opa proteins, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, is required for bacterial pathogenesis. It is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. Recombinant mouse Pkm, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for Pkm active protein
References
Spoden GA., et al. (2009). Exp Cell Res. 315 (16):2765-74; Mazurek S., et al. (2007). Ernst Schering Found Symp Proc. (4):99-124.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60.2 kDa (554aa) Confirmed by MALDI-TOF
NCBI Official Full Name
pyruvate kinase PKM isoform M2
NCBI Official Synonym Full Names
pyruvate kinase, muscle
NCBI Official Symbol
Pkm
NCBI Official Synonym Symbols
Pk3; Pk-2; Pk-3; Pkm2; AA414905; AL024370; AL024424
NCBI Protein Information
pyruvate kinase PKM
UniProt Protein Name
Pyruvate kinase PKM
Protein Family
UniProt Gene Name
Pkm
UniProt Synonym Gene Names
Pk3; Pkm2; Pykm
Uniprot Description
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation ().
Research Articles on Pkm
Similar Products
Product Notes
The Pkm pkm (Catalog #AAA206660) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's Pkm can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Pkm pkm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMPKPHSE AGTAFIQTQQ LHAAMADTFL EHMCRLDIDS APITARNTGI ICTIGPASRS VEMLKEMIKS GMNVARLNFS HGTHEYHAET IKNVREATES FASDPILYRP VAVALDTKGP EIRTGLIKGS GTAEVELKKG ATLKITLDNA YMEKCDENIL WLDYKNICKV VEVGSKIYVD DGLISLQVKE KGADFLVTEV ENGGSLGSKK GVNLPGAAVD LPAVSEKDIQ DLKFGVEQDV DMVFASFIRK AADVHEVRKV LGEKGKNIKI ISKIENHEGV RRFDEILEAS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC NRAGKPVICA TQMLESMIKK PRPTRAEGSD VANAVLDGAD CIMLSGETAK GDYPLEAVRM QHLIAREAEA AIYHLQLFEE LRRLAPITSD PTEAAAVGAV EASFKCCSGA IIVLTKSGRS AHQVARYRPR APIIAVTRNP QTARQAHLYR GIFPVLCKDA VLNAWAEDVD LRVNLAMDVG KARGFFKKGD VVIVLTGWRP GSGFTNTMRV VPVP. It is sometimes possible for the material contained within the vial of "Pkm, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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