Rabbit anti-Human, Mouse ADSSL1 Polyclonal Antibody | anti-ADSSL1 antibody

ADSSL1, NT (ADSSL1, ADSS1, Adenylosuccinate synthetase isozyme 1, Adenylosuccinate synthetase, basic isozyme, Adenylosuccinate synthetase, muscle isozyme, IMP-aspartate ligase 1)

Cat. Num. AAA647047

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ADSSL1; Polyclonal Antibody; NT (ADSSL1; ADSS1; Adenylosuccinate synthetase isozyme 1; Adenylosuccinate synthetase; basic isozyme; muscle isozyme; IMP-aspartate ligase 1); Anti -ADSSL1; anti-ADSSL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ADSSL1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: ADSSL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-131 amino acids from the N-terminal region of human ADSSL1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ADSSL1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (15ug/lane).This demonstrates the ADSSL1 antibody detected ADSSL1 protein (arrow).)

Immunohistochemistry (IHC)

(ADSSL1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADSSL1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-ADSSL1 antibody

Antibodies; Abs to Enzymes; Synthase

NCBI and Uniprot Product Information

• NCBI GI #: 40316944
• NCBI GeneID: 122622
• NCBI Accession #: NP_954634.1
• NCBI GenBank Nucleotide #: NM_199165.1
• UniProt Accession #: Q8N142; Q86TT6; Q8N714
• Molecular Weight: 50,208 Da
• NCBI Official Full Name: adenylosuccinate synthetase isozyme 1 isoform 1
• NCBI Official Synonym Full Names: adenylosuccinate synthase like 1
• NCBI Official Symbol: ADSSL1
• NCBI Protein Information: adenylosuccinate synthetase isozyme 1; adSS 1; AMPSase 1; IMP--aspartate ligase 1; M-type adenylosuccinate synthetase; adenylosuccinate synthetase, basic isozyme; adenylosuccinate synthetase, muscle isozyme
• UniProt Protein Name: Adenylosuccinate synthetase isozyme 1
• Protein Family: Adenylosuccinate synthetase
• UniProt Gene Name: ADSSL1
• UniProt Synonym Gene Names: ADSS1; AMPSase 1; AdSS 1
• UniProt Entry Name: PURA1_HUMAN

NCBI Description

ADSSL1 is a muscle isozyme of adenylosuccinate synthase (EC 6.3.4.4), which catalyzes the initial reaction in the conversion of inosine monophosphate (IMP) to adenosine monophosphate (AMP) (Sun et al., 2005 [PubMed 15786719]).[supplied by OMIM, Dec 2008]

Uniprot Description

Function: Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP

By similarity. HAMAP-Rule MF_03126

Catalytic activity: GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP-Rule MF_03126

Cofactor: Binds 1 magnesium ion per subunit

By similarity. HAMAP-Rule MF_03126

Pathway: Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP-Rule MF_03126

Subunit structure: Homodimer

By similarity. HAMAP-Rule MF_03126

Subcellular location: Cytoplasm Ref.1.

Tissue specificity: Predominantly expressed in skeletal muscle and heart, as well as in several hematopoietic cell lines and solid tumors. Ref.1

Sequence similarities: Belongs to the adenylosuccinate synthetase family.

Sequence caution: The sequence AAH32039.1 differs from that shown. Reason: Frameshift at position 1. The sequence CAD62614.1 differs from that shown. Reason: Erroneous initiation.