PKM2 active protein

PKM2, 1-531aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA203644

• Gene Names: PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: PKM2; 1-531aa; Human; His tag; E Coli (Bioactivity Validated); pyruvate kinase muscle isoform M2; CTHBP; THBP1; p58; M2-PK; Tumor M2-PK; PK2; PK3; PKM; pyruvate kinase; muscle isoform M2 OIP3; TCB; OPA interacting protein 3; Cytosolic thyroid hormone binding protein; Pyruvate kinase 2/3; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle; Thyroid hormone binding protein cytosolic; Tumour Type M2 Pyruvate Kinase; Tumor Type M2 Pyruvate Kinase; PKM2 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSKPHSEAGT AFIQTQQLHA AMADTFLEHM CRLDIDSPPI TARNTGIICT IGPASRSVET LKEMIKSGMN VARLNFSHGT HEYHAETIKN VRTATESFAS DPILYRPVAV ALDTKGPEIR TGLIKGSGTA EVELKKGATL KITLDNAYME KCDENILWLD YKNICKVVEV GSKIYVDDGL ISLQVKQKGA DFLVTEVENG GSLGSKKGVN LPGAAVDLPA VSEKDIQDLK FGVEQDVDMV FASFIRKASD VHEVRKVLGE KGKNIKIISK IENHEGVRRF DEILEASDGI MVARGDLGIE IPAEKVFLAQ KMMIGRCNRA GKPVICATQM LESMIKKPRP TRAEGSDVAN AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAIY HLQLFEELRR LAPITSDPTE ATAVGAVEAS FKCCSGAIIV LTKSGRSAHQ VARYRPRAPI IAVTRNPQTA RQAHLYRGIF PVLCKDPVQE AWAEDVDLRV NFAMNVGKAR GFFKKGDVVI VLTGWRPGSG FTNTMRVVPV P
• Sequence Length: 531

• Applicable Applications for PKM2 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity: > 25,000 pmol/min/ug. One unit will convert 1.0 pmole of phospho (enol)pyruvate to pyruvate per minute at pH 7.5 at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PKM2 active protein

PKM2 is an isoenzyme of the glycolytic enzyme pyruvate kinase. This protein catalyzes the production of pyruvate and ATP from phosphoenolpyruvate. PKM2 interacting with Opa proteins, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, is required for bacterial pathogenesis. It is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. Recombinant human PKM2, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for PKM2 active protein

Cancer

References

Zwerschke W., et al. (2009). Exp Cell Res. 315 (16):2765-74; Mazurek S., et al. (2007). Ernst Schering Found Symp Proc. (4):99-124.;

NCBI and Uniprot Product Information

• NCBI GI #: 33286418
• NCBI GeneID: 5315
• NCBI Accession #: NP_002645
• NCBI GenBank Nucleotide #: NM_002654.5
• UniProt Accession #: P14618; P14786; Q53GK4; Q96E76; Q9BWB5; Q9UCV6; Q9UPF2; A6NFK3; B2R5N8; B3KRY0; B4DFX8; B4DUU6
• Molecular Weight: 60.1 kDa (551aa)
• NCBI Official Full Name: pyruvate kinase PKM isoform a
• NCBI Official Synonym Full Names: pyruvate kinase M1/2
• NCBI Official Symbol: PKM
• NCBI Official Synonym Symbols: PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• NCBI Protein Information: pyruvate kinase PKM
• UniProt Protein Name: Pyruvate kinase PKM
• UniProt Gene Name: PKM
• UniProt Synonym Gene Names: OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1

NCBI Description

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]

Uniprot Description

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.