PKM2 Active Protein | PKM active protein

Human Recombinant PKM2

Cat. Num. AAA844212

• Gene Names: PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: PKM2; Human Recombinant PKM2; Pyruvate kinase; muscle isoform M2; CTHBP; THBP1; p58; M2-PK; Tumor M2-PK; PK2; PK3; PKM.; PKM active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSKPHSEAGT AFIQTQQLHA AMADTFLEHM CRLDIDSPPI TARNTGIICT IGPASRSVET LKEMIKSGMN VARLNFSHGT HEYHAETIKN VRTATESFAS DPILYRPVAV ALDTKGPEIR TGLIKGSGTA EVELKKGATL KITLDNAYME KCDENILWLD YKNICKVVEV GSKIYVDDGL ISLQVKQKGA DFLVTEVENG GSLGSKKGVN LPGAAVDLPA VSEKDIQDLK FGVEQDVDMV FASFIRKASD VHEVRKVLGE KGKNIKIISK IENHEGVRRF DEILEASDGI MVARGDLGIE IPAEKVFLAQ KMMIGRCNRA GKPVICATQM LESMIKKPRP TRAEGSDVAN AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAIY HLQLFEELRR LAPITSDPTE ATAVGAVEAS FKCCSGAIIV LTKSGRSAHQ VARYRPRAPI IAVTRNPQTA RQAHLYRGIF PVLCKDPVQE AWAEDVDLRV NFAMNVGKAR GFFKKGDVVI VLTGWRPGSG FTNTMRVVPV P
• Sequence Length: 605

• Applicable Applications for PKM active protein: SDS-PAGE
• Biological Activity: Specific activity: >5.0 unit/mg.
• Results: >5.0 unit/mg
• Unit Definition: One unit will form 1.0 umol of phosphoenolpyruvate to pyruvate per minute at pH 7.5 at 37 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PKM active protein

Background: Pyruvate kinase is a key enzyme in the glycolytic pathway. The M2 isoenzyme of pyruvate kinase is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. The marker is useful for measuring disease activity, sensitivity to chemotherapy and recurrence. PKM2 interacting with Opa proteins, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, is required for bacterial pathogenesis.

Product Categories/Family for PKM active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 332164775
• NCBI GeneID: 5315
• NCBI Accession #: NP_001193725.1
• NCBI GenBank Nucleotide #: NM_001206796.1
• UniProt Accession #: P14618; P14786; Q53GK4; Q96E76; Q9BWB5; Q9UCV6; Q9UPF2; A6NFK3; B2R5N8; B3KRY0; B4DFX8; B4DUU6
• Molecular Weight: 60.1 kDa (551 aa, 1-531 aa + NT His-Tag)
• NCBI Official Full Name: pyruvate kinase PKM isoform c
• NCBI Official Synonym Full Names: pyruvate kinase, muscle
• NCBI Official Symbol: PKM
• NCBI Official Synonym Symbols: PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• NCBI Protein Information: pyruvate kinase PKM
• UniProt Protein Name: Pyruvate kinase PKM
• Protein Family: Pyruvate kinase
• UniProt Gene Name: PKM
• UniProt Synonym Gene Names: OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
• UniProt Entry Name: KPYM_HUMAN

NCBI Description

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]

Uniprot Description

PKM2: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. Overexpressed in a variety of cancers. Interacts with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. Binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogens is. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms of PKM2 have been reported.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Nucleotide Metabolism - purine; Kinase, other; Carbohydrate Metabolism - pyruvate; Mitochondrial; EC 2.7.1.40

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: extracellular matrix; mitochondrion; cytoplasm; plasma membrane; nucleus; cytosol; vesicle; cilium

Molecular Function: potassium ion binding; protein binding; magnesium ion binding; pyruvate kinase activity; ATP binding

Biological Process: glycolysis; carbohydrate metabolic process; programmed cell death; glucose metabolic process; pathogenesis; phosphorylation