Typical Testing Data/Standard Curve (for reference only)
Mouse Pyruvate kinase isozymes M1/M2 ELISA Kit | Pkm2 elisa kit
Mouse Pyruvate kinase isozymes M1/M2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate kinase isozymes M1/M2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pyruvate kinase isozymes M1/M2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pyruvate kinase isozymes M1/M2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Pyruvate kinase isozymes M1/M2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
PKM2: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. Overexpressed in a variety of cancers. Interacts with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. Binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogens is. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms of PKM2 have been reported.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; EC 2.7.1.40; Kinase, other; Mitochondrial; Nucleotide Metabolism - purine
Cellular Component: cilium; cytoplasm; extracellular matrix; mitochondrion; myelin sheath; nucleus; plasma membrane; vesicle
Molecular Function: ADP binding; ATP binding; catalytic activity; kinase activity; magnesium ion binding; metal ion binding; nucleotide binding; potassium ion binding; protein binding; pyruvate kinase activity; transferase activity
Biological Process: ATP biosynthetic process; glucose metabolic process; glycolysis; liver development; metabolic process; organ regeneration; phosphorylation; programmed cell death; pyruvate biosynthetic process; skeletal muscle regeneration
Research Articles on Pkm2
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Product Notes
The Mouse Pkm2 pkm (Catalog #AAA289101) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA289101 ELISA Kit recognizes Mouse Pkm2. It is sometimes possible for the material contained within the vial of "Pyruvate kinase isozymes M1/M2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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