EphB3 / HEK2 recombinant protein

Recombinant Mouse EphB3 / HEK2 Protein (His tag)

Cat. Num. AAA2546902

• Gene Names: Ephb3; Etk2; HEK2; MDK5; Sek4; Cek10; Tyro6; AW456895
• Purity: > 94 % as determined by SDS-PAGE
• Synonyms: EphB3 / HEK2; Recombinant Mouse EphB3 / HEK2 Protein (His tag); AW456895; Cek10; Etk2; HEK2; MDK5; Sek4; Tyro6; EphB3 / HEK2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 94 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.5
• Sequence Length: 993

• Application Notes: The secreted recombinant mouse EPHB3 consists of 519 amino acids and has a predicted molecular mass of 57 kDa. It migrates as an approximately 63 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Leu 30
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for EphB3 / HEK2 recombinant protein

Background: Ephrin type -B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Description: A DNA sequence encoding the mouse EPHB3 (NP_034273.1) extracellular domain (Met 1-Thr 537) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 33859548
• NCBI GeneID: 13845
• NCBI Accession #: NP_034273.1
• NCBI GenBank Nucleotide #: NM_010143.1
• UniProt Accession #: P54754; Q62214; Q91YS9
• Molecular Weight: 109,662 Da
• NCBI Official Full Name: ephrin type-B receptor 3
• NCBI Official Synonym Full Names: Eph receptor B3
• NCBI Official Symbol: Ephb3
• NCBI Official Synonym Symbols: Etk2; HEK2; MDK5; Sek4; Cek10; Tyro6; AW456895
• NCBI Protein Information: ephrin type-B receptor 3
• UniProt Protein Name: Ephrin type-B receptor 3
• UniProt Gene Name: Ephb3
• UniProt Synonym Gene Names: Etk2; Mdk5; Sek4; mDK-5

Uniprot Description

EphB3: a ubiquitous receptor tyrosine kinase of the Eph family. A receptor for ephrin-B family members: ephrin-B1 and -B2. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels.EphB3 contains 1 sterile alpha motif (SAM) domain and 2 fibronectin type III domains.

Protein type: EC 2.7.10.1; Eph family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 16|16 B1

Cellular Component: integral to plasma membrane

Molecular Function: axon guidance receptor activity; ephrin receptor activity; transmembrane-ephrin receptor activity

Biological Process: angiogenesis; axon guidance; axonal fasciculation; cell migration; central nervous system projection neuron axonogenesis; corpus callosum development; digestive tract morphogenesis; ephrin receptor signaling pathway; palate development; positive regulation of synaptogenesis; protein amino acid autophosphorylation; regulation of axonogenesis; regulation of cell-cell adhesion; regulation of GTPase activity; retinal ganglion cell axon guidance; thymus development; urogenital system development