EphB3 active protein

EphB3 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122841

• Gene Names: Ephb3; Etk2; HEK2; MDK5; Sek4; Cek10; Tyro6; AW456895
• Purity: >94% as determined by SDS-PAGE
• Synonyms: EphB3; EphB3 Protein; Mouse; Recombinant (His Tag); Mouse EphB3/HEK2 Protein (His Tag); AW456895 Protein; Cek10 Protein; Etk2 Protein; HEK2 Protein; MDK5 Protein; Sek4 Protein; Tyro6 Protein; EPH receptor B3; EphB3 active protein

• Host: HEK293 Cells
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr537

• Species: Mouse
• Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse EphB3 at 2ug/ml (100uL/well) can bind mouse EFNB1 with a linear range of 0.1-12.5ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 30
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse EPHB3 (NP_034273.1) extracellular domain (Met 1-Thr 537) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for EphB3 active protein

Background: Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Product Categories/Family for EphB3 active protein

Actin Dynamics Signaling Pathway

References

Bergemann AD, et al. (1998) Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube. Oncogene. 16(4): 471-80.Hock B, et al. (1998) PDZ-domain-mediated interaction of the Eph-related receptor tyrosine kinase EphB3 and the ras-binding protein AF6 depends on the kinase activity of the receptor. Proc Natl Acad Sci U S A. 95(17): 9779-84.Liu X, et al. (2006) EphB3: an endogenous mediator of adult axonal plasticity and regrowth after CNS injury. J Neurosci. 26(12): 3087-101.

NCBI and Uniprot Product Information

• NCBI GI #: 33859548
• NCBI GeneID: 13845
• NCBI Accession #: NP_034273.1
• NCBI GenBank Nucleotide #: NM_010143.1
• UniProt Accession #: P54754; Q62214; Q91YS9
• Molecular Weight: 109,662 Da
• NCBI Official Full Name: ephrin type-B receptor 3
• NCBI Official Synonym Full Names: Eph receptor B3
• NCBI Official Symbol: Ephb3
• NCBI Official Synonym Symbols: Etk2; HEK2; MDK5; Sek4; Cek10; Tyro6; AW456895
• NCBI Protein Information: ephrin type-B receptor 3
• UniProt Protein Name: Ephrin type-B receptor 3
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: Ephb3
• UniProt Synonym Gene Names: Etk2; Mdk5; Sek4; mDK-5

Uniprot Description

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.