Rabbit Influenza A H5N1 Polyclonal Antibody | anti-H5N1 antibody

Influenza A H5N1 (A/Egypt/N05056/2009) Hemagglutinin/HA Antibody, Rabbit PAb, Antigen Affinity Purified

Cat. Num. AAA8119028

• Reactivity: H5N1
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: Influenza A H5N1; Polyclonal Antibody; Influenza A H5N1 (A/Egypt/N05056/2009) Hemagglutinin/HA Antibody; Rabbit PAb; Antigen Affinity Purified; Harvey rat sarcoma viral oncogene homolog; anti-H5N1 antibody

• Host: Rabbit
• Reactivity: H5N1
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Influenza A H5N1 (A/Egypt/N05056/2009) Hemagglutinin/HA
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid. 0.2um filtered solution in PBS

• Applicable Applications for anti-H5N1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-1:5000; ELISA: 1:5000-1:10,000; Antibody's applications haven't been validated with corresponding virus positive samples.
• Immunogen: Recombinant Influenza A H5N1 (A/Egypt/N05056/2009) Hemagglutinin/HA Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Influenza A H5N1 (A/Egypt/N05056/2009) Hemagglutinin/HA. Influenza A H5N1 (A/Egypt/N05056/2009) Hemagglutinin/HA specific IgG was purified by Influenza A H5N1 (A/Egypt/N05056/2009) Hemagglutinin/HA affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Western Blot (WB)

(Anti-Influenza A H5N1 (A/Egypt/N05056/2009) Hemagglutinin / HA polyclonal antibody at 1:1000 dilution.Sample:Influenza A H5N1 (A/Egypt/N05056/2009) Hemagglutinin / HA Recombinant ProteinLane A: 50ngLane B: 10ngSecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

Related Product Information for anti-H5N1 antibody

Background: The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

References

White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018