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Typical Testing Data/Standard Curve (for reference only)

Mouse Ephrin Type B Receptor 3 (EPHB3) RTU ELISA Kit | EPHB3 rtu elisa kit

Mouse Ephrin Type B Receptor 3 (EPHB3) ELISA Kit

Gene Names
Ephb3; Etk2; HEK2; MDK5; Sek4; Cek10; Tyro6; AW456895
Reactivity
Mouse
Synonyms
Ephrin Type B Receptor 3 (EPHB3); Mouse Ephrin Type B Receptor 3 (EPHB3) ELISA Kit; ETK; ETK1; HEK; HEK4; TYRO4; Human embryo kinase; Tyrosine-protein kinase receptor ETK1; EPHB3 rtu elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Sequence Length
993
Assay Type
Sandwich
Samples
Tissue Homogenates or Other Biological Fluids.
Detection Range
0.156-10ng/mL
Sensitivity
0.051ng/mL
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for EPHB3 rtu elisa kit
Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EPHB3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to EPHB3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EPHB3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EPHB3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
109,662 Da
NCBI Official Full Name
ephrin type-B receptor 3
NCBI Official Synonym Full Names
Eph receptor B3
NCBI Official Symbol
Ephb3
NCBI Official Synonym Symbols
Etk2; HEK2; MDK5; Sek4; Cek10; Tyro6; AW456895
NCBI Protein Information
ephrin type-B receptor 3
UniProt Protein Name
Ephrin type-B receptor 3
Protein Family
UniProt Gene Name
Ephb3
UniProt Synonym Gene Names
Etk2; Mdk5; Sek4; mDK-5

Uniprot Description

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.

Research Articles on EPHB3

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Product Notes

The Mouse EPHB3 ephb3 (Catalog #AAA459076) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA459076 RTU ELISA Kit recognizes Mouse EPHB3. It is sometimes possible for the material contained within the vial of "Ephrin Type B Receptor 3 (EPHB3), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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