Testing Data
Von Willebrand Factor/vWF ELISA Pair Set | vWF elisa pair set
Von Willebrand Factor/vWF Matched ELISA Antibody Pair Set, Human
Synonyms
Von Willebrand Factor/vWF; Von Willebrand Factor/vWF Matched ELISA Antibody Pair Set; Human; Human VWF ELISA Pair Set; von Willebrand factor; F8VWF Matched ELISA Antibody Pair Set; Von Willebrand Factor Matched ELISA Antibody Pair Set; VWD Matched ELISA Antibody Pair Set; vWF elisa pair set
Specificity
Quantitative determination of Human Von Willebrand Factor/vWF
Assay Type
Solid Phase Sandwich ELISA
Detection Range
109.38-7000pg/mL
Sensitivity
312.5pg/ml
Materials Provided
1. Capture Antibody 1 mg/mL of mouse anti-Human VWF monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human VWF monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.25ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 180 ng of recombinant Human VWF. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 7000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human VWF monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.25ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 180 ng of recombinant Human VWF. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 7000 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for vWF elisa pair set
Von Willebrand Factor (VWF) is a multimeric glycoprotein involved in hemostasis in blood, binds receptors on the surface of platelets and in connective tissue, thereby mediating the adhesion of platelets to sites of vascular injury. From studies it appears that VWF protein uncoils under these circumstances, decelerating passing platelets. VWF protein is deficient or defective in von Willebrand disease (VWD) and is involved in a large number of other diseases, including thrombosis, thrombotic thrombocytopenic purpura, Stroke, Heyde's syndrome, possibly hemolytic-uremic syndrome and so on.
This Von Willebrand Factor/vWF Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Von Willebrand Factor/vWF. It contains Human Von Willebrand Factor/vWF capture antibody, Human Von Willebrand Factor/vWF detector antibody (HRP) and a highly purified CHO Stable-expressed recombinant Human Von Willebrand Factor/vWF protein.
This Von Willebrand Factor/vWF Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Von Willebrand Factor/vWF. It contains Human Von Willebrand Factor/vWF capture antibody, Human Von Willebrand Factor/vWF detector antibody (HRP) and a highly purified CHO Stable-expressed recombinant Human Von Willebrand Factor/vWF protein.
References
Sadler JE. (1998) Biochemistry and genetics of von Willebrand factor. Annu Rev Biochem, 67: 395-424.Batlle J, et al. (2009) Von Willebrand factor/factor VIII concentrates in the treatment of von Willebrand disease. Blood Coagul Fibrinolysis. 20(2):89-100.Sadler JE. (2009) von Willebrand factor assembly and secretion. J Thromb Haemost. 7 Suppl 1:24-7.Auton M, et al. (2010) The mechanism of VWF-mediated platelet GPIbalpha binding. Biophys J. 99(4):1192-201.Bowen DJ. (2010) Sugar targets VWF for the chop. Blood. 115(13):2565.Lpez JA, et al. (2010) VWF self-association: more bands for the buck. Blood. 116(19):3693-4.
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Product Notes
The vWF (Catalog #AAA8118570) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Von Willebrand Factor/vWF, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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