Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Synapsin-2 (SYN2) Standard Curve.)
Rat Synapsin-2 (SYN2) ELISA Kit | SYN2 elisa kit
Rat Synapsin-2 (SYN2) ELISA Kit
Reactivity
Rat
Synonyms
Synapsin-2 (SYN2); Rat Synapsin-2 (SYN2) ELISA Kit; SYN2; SYNII; SYNIIa; SYNIIb; SYN2 elisa kit
Reactivity
Rat
Specificity
Rat Synapsin-2 (SYN2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SYN2. No significant cross-reactivity or interference between Rat SYN2 and analogues was observed.
Sequence Length
586
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Synapsin-2 (SYN2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Synapsin-2 (SYN2) Standard Curve.)
Related Product Information for SYN2 elisa kit
Description: This Rat Synapsin-2 (SYN2) ELISA Kit employs a two-site sandwich ELISA to quantitate SYN2 in samples. An antibody specific for SYN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SYN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Synapsin II is a member of the synapsin family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family encodes a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal. Synapsin II the collective name for two proteins, Synapsin IIa and Synapsin IIb, with Synapsin IIa being the larger of the two isoforms. Their apparent molecular weights are 74,000 and 55,000 per SDS gel electrophoresis.
Background: Synapsin II is a member of the synapsin family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family encodes a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal. Synapsin II the collective name for two proteins, Synapsin IIa and Synapsin IIb, with Synapsin IIa being the larger of the two isoforms. Their apparent molecular weights are 74,000 and 55,000 per SDS gel electrophoresis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Synapsin-2
NCBI Official Synonym Full Names
synapsin II
NCBI Official Symbol
Syn2
NCBI Protein Information
synapsin-2
UniProt Protein Name
Synapsin-2
Protein Family
UniProt Gene Name
Syn2
UniProt Entry Name
SYN2_RAT
NCBI Description
neuronal phosphoprotein that coats synaptic vesicles; binds to the cytoskeleton; may be important for vesicle trafficking to synapses and for the regulation of neurotransmitter release [RGD, Feb 2006]
Uniprot Description
SYN2: neuronal phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal and to the cytoskeleton. May function in the regulation of neurotransmitter release and synaptogenesis. Mutations in this gene may be associated with abnormal presynaptic function and schizophrenia. Alternative splicing results in two isoforms.
Protein type: Vesicle
Cellular Component: cell junction; myelin sheath; synapse; synaptic vesicle; synaptic vesicle membrane; terminal button
Molecular Function: ATP binding; calcium-dependent protein binding; catalytic activity; cytoskeletal protein binding; protein binding
Biological Process: neurotransmitter secretion; regulation of neurotransmitter secretion
Research Articles on SYN2
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Product Notes
The Rat SYN2 syn2 (Catalog #AAA9711755) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711755 ELISA Kit recognizes Rat SYN2. It is sometimes possible for the material contained within the vial of "Synapsin-2 (SYN2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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