Human Contactin-5 ELISA Kit | CNTN5 elisa kit

Human Contactin-5 PicoKine ELISA Kit

Cat. Num. AAA1752571

• Gene Names: CNTN5; NB-2; HNB-2s
• Reactivity: Human
• Synonyms: Contactin-5; Human Contactin-5 PicoKine ELISA Kit; Human Contactin-5; contactin 5; Neural recognition molecule NB-2; hNB-2; CNTN5; CNTN5 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CNTN5
• Sequence Length: 1100

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CNTN5 elisa kit

Principle of the Assay: The Picokine™ Human CNTN5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CNTN5 with a 96-well strip plate that is pre-coated with antibody specific for CNTN5. The detection antibody is a biotinylated antibody specific for CNTN5. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human CNTN5 with immunogen: Expression system for standard: NSO, Immunogen sequence: E19-S1072. The kit is analytically validated with ready to use reagents. To measure Human CNTN5, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CNTN5 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CNTN5 in the sample.!!Background/Introduction: Contactin-5 is a protein that in humans is encoded by the CNTN5 gene. It is mapped to 11q22.1. The protein encoded by this gene is a member of the immunoglobulin superfamily, and contactin family, which mediate cell surface interactions during nervous system development. This protein is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 343168755
• NCBI GeneID: 53942
• NCBI Accession #: NP_001230199.1
• NCBI GenBank Nucleotide #: NP_001230199.1
• UniProt Accession #: O94779
• NCBI Official Full Name: contactin-5 isoform 1
• NCBI Official Synonym Full Names: contactin 5
• NCBI Official Symbol: CNTN5
• NCBI Official Synonym Symbols: NB-2; HNB-2s
• NCBI Protein Information: contactin-5
• UniProt Protein Name: Contactin-5
• Protein Family: Contactin
• UniProt Gene Name: CNTN5
• UniProt Synonym Gene Names: hNB-2
• UniProt Entry Name: CNTN5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunoglobulin superfamily, and contactin family, which mediate cell surface interactions during nervous system development. This protein is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CNTN5: Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity in the cerebral cortical neurons but not in hippocampal neurons. Probably involved in neuronal activity in the auditory system. Belongs to the immunoglobulin superfamily. Contactin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 11q22.1

Cellular Component: cytoplasm; plasma membrane

Biological Process: sensory perception of sound; cell adhesion