CNTN5 active protein

CNTN5 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120325

• Gene Names: CNTN5; NB-2; HNB-2s
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CNTN5; CNTN5 Protein; Human; Recombinant (His Tag); Human Contactin 5/CNTN5 Protein (His Tag); HNB-2s Protein; NB-2 Protein; contactin 5; CNTN5 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln1059

• Species: Human
• Activity: Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to CNTN5 coated plates (12.5ug/ml and 100uL/well), approximately 30%-50% will adhere specifically after 60 minutes at 37 degree C.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mature form of human CNTN5 (NP_055176.1)(Met 1-Gln 1059) with a C-terminal polyhistidine tag was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CNTN5 active protein

Background: Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed mainly in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2(TAG-1), Contactin-3(BIG-1), BIG-2, Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-5, also known as NB-2, is one of the neural recognition molecules in the contactin subgroup. Contactin-5 is expressed in brain and kidney and at very low level in placenta. In brain, Contactin-5 is highly expressed in the occipital lobe, amygdala, cerebral cortex, frontal lobe, thalamus and temporal lobe. Mice deficient in the Contactin-5 gene exhibit aberrant responses to acoustic stimuli. Contactin-5 may play a role in maturation of glutamatergic synapses in the brainstem during the final stages of auditory development. Contactin-5 gene may contribute to human neurological disorders.

References

Kamei Y, et al. (2000) Human NB-2 of the contactin subgroup molecules: chromosomal localization of the gene (CNTN5) and distinct expression pattern from other subgroup members. Genomics 69(1):113-9.Ogawa J, et al. (2001) Neural recognition molecule NB-2 of the contactin/F3 subgroup in rat: Specificity in neurite outgrowth-promoting activity and restricted expression in the brain regions. J Neurosci Res. 65(2):100-10.Li H, et al. (2003) Aberrant responses to acoustic stimuli in mice deficient for neural recognition molecule NB-2. Eur J Neurosci. 17(5):929-36.Shimoda Y, et al. (2009) Contactins: Emerging key roles in the development and function of the nervous system. Cell adhesion & migration 3(1):64-70.Toyoshima M, et al. (2009) Preferential localization of neural cell recognition molecule NB-2 in developing glutamatergic neurons in the rat auditory brainstem. J Comp Neurol. 513(4):349-62.

NCBI and Uniprot Product Information

• NCBI GI #: 7657359
• NCBI GeneID: 53942
• NCBI Accession #: NP_055176.1
• NCBI GenBank Nucleotide #: NM_014361.3
• UniProt Accession #: O94779; O94780; Q49AF3; A1L4P0; B7ZM07; E9PKE8
• Molecular Weight: 100,327 Da
• NCBI Official Full Name: contactin-5 isoform 1
• NCBI Official Synonym Full Names: contactin 5
• NCBI Official Symbol: CNTN5
• NCBI Official Synonym Symbols: NB-2; HNB-2s
• NCBI Protein Information: contactin-5
• UniProt Protein Name: Contactin-5
• Protein Family: Contactin
• UniProt Gene Name: CNTN5
• UniProt Synonym Gene Names: hNB-2
• UniProt Entry Name: CNTN5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunoglobulin superfamily, and contactin family, which mediate cell surface interactions during nervous system development. This protein is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CNTN5: Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity in the cerebral cortical neurons but not in hippocampal neurons. Probably involved in neuronal activity in the auditory system. Belongs to the immunoglobulin superfamily. Contactin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 11q22.1

Cellular Component: cytoplasm; plasma membrane

Biological Process: sensory perception of sound; cell adhesion